This study was conducted to determine the effects of three organosilicone-based and six conventional organic adjuvants on the absorption and translocation of 14C-glyphosate in guineagrass and redroot pigweed. The organosilicone adjuvants produced rapid absorption of the 14C-glyphosate into the redroot pigweed leaves, reaching maximum absorption within 0.5–1.0 h after application. The conventional adjuvants produced slower absorption of the 14C-glyphosate, as the maximum absorption was not achieved until at least 24 h after application in redroot pigweed, remaining similar until 72 h. In guineagrass, the maximum absorption of the glyphosate was earlier than 24 h with the organosilicone-based adjuvants, compared with longer times for the conventional adjuvants. The organosilicone-based adjuvants also increased the glyphosate translocation in redroot pigweed, but not in guineagrass. Organosilicone adjuvants have the potential to provide greater rainfastness to glyphosate on redroot pigweed and, to a lesser extent, on guineagrass.