Association of a single nucleotide polymorphism in the secreted frizzled-related protein 4 (sFRP4) gene with bone mineral density
Article first published online: 10 AUG 2004
Geriatrics & Gerontology International
Volume 4, Issue 3, pages 175–180, September 2004
How to Cite
Fujita, M., Urano, T., Shiraki, M., Momoeda, M., Tsutsumi, O., Hosoi, T., Orimo, H., Ouchi, Y. and Inoue, S. (2004), Association of a single nucleotide polymorphism in the secreted frizzled-related protein 4 (sFRP4) gene with bone mineral density. Geriatrics & Gerontology International, 4: 175–180. doi: 10.1111/j.1447-0594.2004.00249.x
- Issue published online: 10 AUG 2004
- Article first published online: 10 AUG 2004
- Accepted for publication 23 March 2004.
- bone mineral density;
- secreted frizzled-related protein 4;
- single nucleotide polymorphism
Background: Wnt-β-catenin signaling pathway is involved in the regulation of bone mineral density (BMD). Secreted frizzled-related protein (sFRP) 4 that antagonize Wnt signals may modulate Wnt-β-catenin signaling pathway in the bone. Therefore, we analyzed expression of sFRP4 mRNA in primary osteoblasts and the association of a single nucleotide polymorphism (SNP) in the sFRP4 gene with BMD.
Methods: Expression levels of sFRP4 mRNA were analyzed during the culture course of rat primary osteoblasts. Association of a SNP in the sFRP4 gene at Arg262 (CGC to CGT) with BMD was examined in 372 healthy post-menopausal Japanese women.
Results: sFRP4 mRNA was detected and increased during the differentiation of rat primary osteoblasts. As an association study of the SNP in the sFRP4 gene, the subjects without the T allele (CC; n = 129) had significantly higher lumbar BMD than the subjects bearing at least one T allele (TT + CT; n = 243) (Z score; 0.054 versus−0.324; P = 0.0188).
Conclusion: sFRP4 mRNA was expressed and regulated in primary osteoblasts. A genetic variation at the sFRP4 gene locus is associated with BMD, suggesting an involvement of sFRP4 gene in the bone metabolism.