Inhibition of matrix metalloproteinase-2 secretion and invasion by human ovarian cancer cell line SK-OV-3 with lysine, proline, arginine, ascorbic acid and green tea extract
Article first published online: 17 MAR 2006
Journal of Obstetrics and Gynaecology Research
Volume 32, Issue 2, pages 148–154, April 2006
How to Cite
Roomi, M. W., Ivanov, V., Kalinovsky, T., Niedzwiecki, A. and Rath, M. (2006), Inhibition of matrix metalloproteinase-2 secretion and invasion by human ovarian cancer cell line SK-OV-3 with lysine, proline, arginine, ascorbic acid and green tea extract. Journal of Obstetrics and Gynaecology Research, 32: 148–154. doi: 10.1111/j.1447-0756.2006.00389.x
- Issue published online: 17 MAR 2006
- Article first published online: 17 MAR 2006
- Received: July 1 2005. Accepted: January 20 2006.
- antitumor effect;
- matrix metalloproteinases;
- ovarian cancer;
Aims: Based on the poor prognosis associated with ovarian cancer and reported anticancer properties of specific nutrients, we investigated the effect of a nutrient mixture (NM) containing lysine, proline, arginine, ascorbic acid and epigallocatechin gallate on human ovarian cancer cells SK-OV-3 by measuring: cell proliferation, modulation of matrix metalloproteinase (MMP)-2 and -9 expression, and cancer cell invasive potential.
Methods: Cell proliferation was evaluated by MTT assay, MMP activity by gelatinase zymography, and invasion through Matrigel.
Results: Human ovarian cancer cell growth was not significantly affected by the NM. Zymography demonstrated inhibition of MMP-2 secretion in a dose-dependent fashion with virtual total inhibition at 50 µg/mL NM concentration. Invasion of human ovarian cancer cells through Matrigel decreased in a dose-dependent fashion, with 90% inhibition at 500 µg/mL NM and 100% inhibition at 1000 µg/mL NM (P < 0.0001).
Conclusion: The combination of lysine, proline, arginine, ascorbic acid and green tea extract tested inhibited critical steps in cancer development and spread, such as MMP expression and invasion, indicating its potential as a treatment modality against ovarian cancer.