Sequence accession numbers: Partial sequences of 16S rRNA genes representative of each phylotype from the five subjects have been deposited in GenBank (Accession numbers EF653402-653428).
Preliminary characterization of vaginal microbiota in healthy Chinese women using cultivation-independent methods
Article first published online: 18 MAR 2009
© 2009 The Authors. Journal compilation © 2009 Japan Society of Obstetrics and Gynecology
Journal of Obstetrics and Gynaecology Research
Volume 35, Issue 3, pages 525–532, June 2009
How to Cite
Shi, Y., Chen, L., Tong, J. and Xu, C. (2009), Preliminary characterization of vaginal microbiota in healthy Chinese women using cultivation-independent methods. Journal of Obstetrics and Gynaecology Research, 35: 525–532. doi: 10.1111/j.1447-0756.2008.00971.x
- Issue published online: 10 JUN 2009
- Article first published online: 18 MAR 2009
- Received: March 6 2008.Accepted: August 25 2008.
- 16S rRNA;
Aim: The normal microbiota plays an important role in maintaining the vaginal health of women. The aim of this study was to characterize the numerically dominant populations of vaginal microbial communities in Chinese women.
Methods: Genomic DNA was isolated from samples of vaginal swabs of five healthy women, 16S ribosomal ribonucleic acid libraries were constructed and the sequences of 300 clones from each library were determined and subjected to phylogenetic analyses. The phylotypes were classified based on sequence similarity to known organisms.
Results: All vaginal microbial communities were dominated by the Lactobacillus species, and they constituted from 96.4% to 100% of the clones sequenced. Sequences homologous to L. crispatus and L. iners were most common, which were highly homologous with those of Caucasian women in the USA.
Conclusions: The numerically dominant microbial populations in healthy Chinese women's vaginas were L. crispatus and L. iners. The limited number of Lactobacillus phylotypes found in the human vagina suggested that host factors have a role in the selection of specific organisms, which have specific characteristics that allow them to successfully colonize the vagina.