SHP-2 phosphatase promotes cervical cancer cell proliferation through inhibiting interferon-β production
Article first published online: 13 AUG 2012
© 2012 The Authors. Journal of Obstetrics and Gynaecology Research © 2012 Japan Society of Obstetrics and Gynecology
Journal of Obstetrics and Gynaecology Research
Volume 39, Issue 1, pages 272–279, January 2013
How to Cite
Meng, F., Zhao, X. and Zhang, S. (2013), SHP-2 phosphatase promotes cervical cancer cell proliferation through inhibiting interferon-β production. Journal of Obstetrics and Gynaecology Research, 39: 272–279. doi: 10.1111/j.1447-0756.2012.01952.x
- Issue published online: 7 JAN 2013
- Article first published online: 13 AUG 2012
- Received: November 8 2011.; Accepted: May 10 2012.
- cell proliferation;
- cervical cancer;
- SH2-containing protein tyrosine phosphatase 2;
- type I interferon β
Aim: The aim of this study was to explore the effect of Src-homology-2-domain-containing protein, tyrosine phosphatase 2 (SHP-2) on the proliferation of cervical cancer cells.
Material and Methods: A total of 45 patients with cervical cancer (stage I–III), 32 with cervical intraepithelial neoplasia and 20 healthy subjects were consecutively recruited. The levels of SHP-2 and interferon (IFN)-β expression in cervical tissues were characterized by immunohistochemistry and statistically analyzed by logistic regression. Following knockdown of SHP-2 expression by a siRNA or pre-treatment with a specific peptide, the effect of SHP-2 expression in THP-1 cells on the growth and survival of SiHa cells and on IFN-β production was determined by co-culture assays, 3-(4,5)-dimethylthiazol (-z-y 1)-3,5-diphenyltetrazolium bromide, and enzyme immunosorbent assay.
Results: The levels of SHP-2 expression in cervical cancer tissues were significantly higher than that in cervical intraepithelial neoplasia and uterine myoma tissues (P < 0.05, respectively), and negatively correlated with the levels of IFN-β expression in these tissues (R = −0.582, P < 0.05). Knockdown of SHP-2 expression with SHP-2 siRNA or treatment with the SHP-2-specific blocking peptide in THP-1 cells significantly increased the production of IFN-β (P < 0.05, respectively) and inhibited the proliferation of SiHa cells in a co-culture system of THP-1 and SiHa cells (P < 0.05, respectively).
Conclusions: SHP-2 phosphatase promotes cervical cancer cell proliferation through inhibiting IFN-β production.