Cloning and Characterization of the Rat Gene Encoding GAP-43

Authors

  • Ed Grabczyk,

    1. Developmental Biology Laboratory, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, and Howard Hughes Medical Institute, Boston, MA, USA
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  • Mauricio X. Zuber,

    1. Developmental Biology Laboratory, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, and Howard Hughes Medical Institute, Boston, MA, USA
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  • Howard J. Federoff,

    1. Developmental Biology Laboratory, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, and Howard Hughes Medical Institute, Boston, MA, USA
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    • 1

      Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA

  • Shi-Chung Ng,

    1. Developmental Biology Laboratory, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, and Howard Hughes Medical Institute, Boston, MA, USA
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    • 2

      Squibb Institute for Scientific Research, Princeton, NJ, USA

  • Alison Pack,

    1. Developmental Biology Laboratory, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, and Howard Hughes Medical Institute, Boston, MA, USA
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  • Mark C. Fishman

    Corresponding author
    1. Developmental Biology Laboratory, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, and Howard Hughes Medical Institute, Boston, MA, USA
      Correspondence to: Mark C. Fishman, Developmental Biology Laboratory, Massachusetts General Hospital, Boston, MA 02114, USA
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Correspondence to: Mark C. Fishman, Developmental Biology Laboratory, Massachusetts General Hospital, Boston, MA 02114, USA

Abstract

GAP-43 is a gene expressed only in the nervous system. The protein product is believed to be important to neuronal growth and plasticity. Most, and likely all, neurons express high levels of GAP-43 during periods of neurite elongation. To initiate studies of GAP-43 gene regulation we have cloned the rat gene encoding GAP-43. The GAP-43 gene includes three exons. The first exon encodes only the amino terminal 10 amino acids, which corresponds to the membrane targeting domain of GAP-43. The second exon encodes a putative calmodulin binding domain and a protein kinase C phosphorylation site. The 5′-flanking sequence is unusual in that it lacks CAAT or TATA elements, and directs RNA transcription initiation from several sites. Some of the transcription start sites are used to a different degree in the central and peripheral nervous systems.

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