Get access

Axotomy-induced Changes in Ca2+ Homeostasis in Rat Sympathetic Ganglion Cells

Authors

  • Maria V. Sánchez-Vives,

    1. Instituto de Neurociencias, Universidad de Alicante, Apartado 374, 03080 Alicante, Spain
    2. Departmento de Fisiología, Universidad de Alicante, Alicante, Spain
    Search for more papers by this author
    • 3

      The Rockefeller University, New York, USA

  • Miguel Valdeolmillos,

    1. Instituto de Neurociencias, Universidad de Alicante, Apartado 374, 03080 Alicante, Spain
    2. Departmento de Fisiología, Universidad de Alicante, Alicante, Spain
    Search for more papers by this author
  • Salvador Martínez,

    1. Instituto de Neurociencias, Universidad de Alicante, Apartado 374, 03080 Alicante, Spain
    2. Departmento de Ciencias Morfológicas, Universidad de Murcia, Murcia, Spain
    Search for more papers by this author
  • Roberto Gallego

    Corresponding author
    1. Instituto de Neurociencias, Universidad de Alicante, Apartado 374, 03080 Alicante, Spain
    2. Departmento de Fisiología, Universidad de Alicante, Alicante, Spain
      R. Gallego, as above
    Search for more papers by this author

R. Gallego, as above

Abstract

Some of the marked biochemical and electrophysiological changes provoked by section of the axon in mature neurons suggest that the intracellular calcium concentration ([Ca2+]i) may be increased. We have measured the [Ca2+]i using the fluorescent indicator Indo-1 microinjected into rat superior cervical ganglion neurons. No differences in resting [Ca2+]i levels were found between control neurons and cells which had been axotomized 7–10 days before. However, the rise in [Ca2+]i evoked by orthodromic or antidromic stimulation and the recovery after the stimulating train were considerably slower in axotomized neurons than in control cells. We also found that the number of calbindin-D28k-immunopositive cells in the ganglion increases after axotomy, which could be related to the observed differences in calcium homeostasis.

Get access to the full text of this article

Ancillary