Flip and Flop Variants of AMPA Receptors in the Rat Lumbar Spinal Cord

Authors

  • T. R. Tölle,

    Corresponding author
    1. Max Planck Institute of Psychiatry, Clinical Institute, Clinical Neuropharmacology, Kraepelinstrasse 10, 80804 Munich, Germany
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  • A. Berthele,

    1. Max Planck Institute of Psychiatry, Clinical Institute, Clinical Neuropharmacology, Kraepelinstrasse 10, 80804 Munich, Germany
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  • W. Zieglgänsberger,

    1. Max Planck Institute of Psychiatry, Clinical Institute, Clinical Neuropharmacology, Kraepelinstrasse 10, 80804 Munich, Germany
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  • P. H. Seeburg,

    1. Centre for Molecular Biology (ZMBH), University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
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  • W. Wisden

    1. Centre for Molecular Biology (ZMBH), University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
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    • 1

      Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 2QH, UK


Correspondence to: Thomas R. Tölle, as above

Abstract

The expression of eight messenger RNA splice forms encoding the Flip and Flop variants of AMPA receptor subunits GluR-A to -D in the rat lumbar spinal cord was examined by in situ hybridization using specific oligonucleotides. In the dorsal horn (laminae I, II and III) the predominant mRNA was GluR-B Flip. Much lower levels of GluR-A Flip were found in lamina I and in superficial parts of lamina II outer. In the ventral horn, motor neurons expressed mainly GluR-B Flip, GluR-C Flip and Flop, and GluR-D Flip. Serial sectioning through large motor neurons indicated that a given cell contained, for example, both GluR-C Flip and Flop splice types.

Ancillary