A Single-cell Study of the Axonal Projections Arising from the Posterior Intralaminar Thalamic Nuclei in the Rat

Authors

  • Martin Deschênes,

    Corresponding author
    1. Centre de Recherche en Neurobiologie, Hôpital de l'Enfant-Jésus, Université Laval, 1401, 18ème rue, Québec, Canada G1J 1Z4
    Search for more papers by this author
  • Jacques Bourassa,

    1. Centre de Recherche en Neurobiologie, Hôpital de l'Enfant-Jésus, Université Laval, 1401, 18ème rue, Québec, Canada G1J 1Z4
    Search for more papers by this author
  • Van Diep Doan,

    1. Centre de Recherche en Neurobiologie, Hôpital de l'Enfant-Jésus, Université Laval, 1401, 18ème rue, Québec, Canada G1J 1Z4
    Search for more papers by this author
  • André Parent

    1. Centre de Recherche en Neurobiologie, Hôpital de l'Enfant-Jésus, Université Laval, 1401, 18ème rue, Québec, Canada G1J 1Z4
    Search for more papers by this author

Correspondence to: Dr Martin Deschênes, as above

Abstract

Thalamostriatal projections arising from the posterior intralaminar nuclei (Pl; the parafascicular nucleus and the adjacent caudalmost part of the posterior thalamic group) were studied in rats by tracing the axons of small pools of neurons labelled anterogradely with biocytin. Thirteen Pl cells were also stained by juxtacellular application of the tracer. Relay cells of Pl nuclei have a morphology that differs radically from the classical descriptions of the bushy cells which represent the main neuronal type of the sensory thalamic relay nuclei. Pl cells have ovoid or polygonal somata of ˜20–25 μm, from which emerge four or five thick, long and poorly branched dendrites bearing spines and filamentous appendages; their dendritic domains extend for up to 1.5 mm. Before leaving the nucleus 20% of axons give off collaterals that ramify locally. All axons course through the thalamic reticular nucleus, where they emit one or two poorly branched collaterals, traverse the globus pallidus, where they also distribute collaterals, and arborize massively in the striatum and sparsely in the cerebral cortex. At the striatal level four or five collaterals leave the main axon and terminate in patches scattered dorsoventrally within a rostrocaudally oriented slab. As revealed by calbindin D-28k immunohistochemistry, only the matrix compartment receives terminations from Pl axons. The cortical branch forms small terminal puffs centred upon layer VI of the motor cortex. Before entering the striatum some axons of the parafascicular nucleus give rise to descending collaterals that arborize in the entopeduncular nucleus, in the subthalamic nucleus and in the vicinity of the red nucleus. Other axons arising from the caudal part of the posterior group send descending branches only to the entopeduncular nucleus. These findings show that Pl cells belong to a distinct category of thalamic relay neurons which, beside their massive projection to the striatum, also distribute collaterals to other components of the basal ganglia. Moreover, these results provide the first direct evidence that virtually all Pl cells project to both striatum and cerebral cortex. Finally, it is proposed on the basis of morphological, histochemical and hodological criteria that the caudal part of the posterior thalamic group in the rat is homologous to the suprageniculate-limitans nuclei of cats and primates.

Ancillary