Get access

The Distribution of Brain-derived Neurotrophic Factor and its Receptor trkB in Parvlbumin-containing Neurons of the Rat Visual Cortex

Authors

  • Alessandro Cellerino,

    Corresponding author
    1. Istituto di Neurofisiologia del CNR, via S. Zeno 51, 1-561 126 Pisa, Italy and Scuola Normale Superiore, Pisa, Italy
      Alessandro Cellerino, Istituto di Neurofisiologia del CNR. Via S. Zeno 51, 56127 Pisa, Italy
    Search for more papers by this author
  • Lamberto Maffei,

    1. Istituto di Neurofisiologia del CNR, via S. Zeno 51, 1-561 126 Pisa, Italy and Scuola Normale Superiore, Pisa, Italy
    Search for more papers by this author
  • Luciano Domenici

    1. Istituto di Neurofisiologia del CNR, via S. Zeno 51, 1-561 126 Pisa, Italy and Scuola Normale Superiore, Pisa, Italy
    Search for more papers by this author

Alessandro Cellerino, Istituto di Neurofisiologia del CNR. Via S. Zeno 51, 56127 Pisa, Italy

Abstract

We analysed the distribution of brain-derived neurotrophic factor (BDNF) and its receptor trkB in the adult rat visual cortex, paying particular attention to a GABAergic neuronal subpopulation—the parvalburnin-positive cells. We found expression of trkB in the cell body and apical dendrite of pyramidal neurons and in the cell body of non-pyramidal neurons. Double labelling experiments revealed extensive colocalization of parvalbumin and trkB immunoreactivity in non-pyramidal neurons. Interestingly, the trkB-positive pyramidal neurons appeared surrounded by parvalbumin-labelled boutons. The use of double immunohistochemistry and in situ hybridization histochemistry showed that parvalbumin-positive neurons express trkB mRNA. BDNF rnRNA was found in several cells. Coexpression of BDNF mRNA and parvalbumin immunoreactivity was extremely rare. These data strongly suggest that BDNF synthesized by cortical neurons acts as a postsynaptically derived factor for parvalbumin-positive neurons in the adult rat visual cortex.

Get access to the full text of this article

Ancillary