Analysis of the Ion Channel Complement of the Rat Oligodendrocyte Progenitor in a Commonly Studied In vitro Preparation

Authors

  • A. V. Williamson,

    1. The MRC Centre for Brain Repair, MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
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    • 3

      UCHSC Department of Pharmacology, Campus Box B-138, 4200 East Ninth Avenue, Denver, Colorado 80262, USA

  • D. A. S. Compston,

    1. The MRC Centre for Brain Repair, MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
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  • A. D. Randall

    Corresponding author
    1. Neurobiology Division, MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    • A. D. Randall, as above

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Abstract

We have analysed the ion channel complement of the oligodendrocyte-type 2 astrocyte (O-2A) glial cell progenitor obtained from the commonly studied neonatal rat mixed brain preparation. Ionic currents, in O-2A progenitors identified on both morphological and immunological grounds, were recorded using the whole-cell variant of the patch-clamp technique. The cells had an average resting membrane potential close to -50 mV and fired single action potentials in response to suprathreshold current injections. Using voltage-clamp methods we were able to identify and characterize a voltage-activated TTX-sensitive Na+ current, two classes of voltage-activated outward K+ currents, an inactivating inwardly rectifying K+ current, a voltage-activated Cl- current and at least three classes of Ca2+ current.

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