Expression of GABAA Receptor Subunit mRNAs by Layer V Pyramidal Cells of the Rat Primary Visual Cortex



The expression of the GABAA receptor subunit mRNAs by layer V pyramidal neurons of the primary visual cortex and cerebellar Purkinje cells was analysed by single-cell reverse transcription of the mRNAs and amplification of the resulting cDNAs by the polymerase chain reaction. Neurons were identified by infrared Videomicroscopy, and GABAA-mediated miniature inhibitory postsynaptic currents were recorded. In Purkinje cells, α1, β2, β3, γ2s and γ2L subunit mRNAs were detected within a single cell. In layer V pyramidal cells, a total of ten GABAA receptor subunit mRNAs could be detected, with a mean of seven subunit mRNAs per cell, suggesting GABAA receptor heterogeneity within a single pyramidal cell.