Get access

Seizures preferentially stimulate proliferation of radial glia-like astrocytes in the adult dentate gyrus: functional and immunocytochemical analysis

Authors

  • Kerstin Hüttmann,

    1. Experimental Neurobiology, Neurosurgery, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany
    Search for more papers by this author
    • *

      K.H., M.S. and A.W. contributed equally to this work.

  • Matthew Sadgrove,

    1. Division of Clinical Neurosciences, University of Southampton, Room 6207, Biomedical Sciences Building, Bassett Crescent East, Southampton SO16 7PX, UK
    Search for more papers by this author
    • *

      K.H., M.S. and A.W. contributed equally to this work.

  • Anke Wallraff,

    1. Experimental Neurobiology, Neurosurgery, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany
    Search for more papers by this author
    • *

      K.H., M.S. and A.W. contributed equally to this work.

  • Stefan Hinterkeuser,

    1. Experimental Neurobiology, Neurosurgery, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany
    Search for more papers by this author
  • Frank Kirchhoff,

    1. MPI for Experimental Medicine, Herrmann-Rein-Str. 3, 37075 Göttingen, Germany
    Search for more papers by this author
  • Christian Steinhäuser,

    1. Experimental Neurobiology, Neurosurgery, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany
    Search for more papers by this author
  • William P. Gray

    1. Division of Clinical Neurosciences, University of Southampton, Room 6207, Biomedical Sciences Building, Bassett Crescent East, Southampton SO16 7PX, UK
    Search for more papers by this author

: Dr William Gray or Dr Christian Steinhäuser as above.
E-mail: w.p.gray@soton.ac.uk or Christian.Steinhaeuser@ukb.uni-bonn.de

Abstract

Kainate-induced seizures increase hippocampal neurogenesis. Glial fibrillary acidic protein-positive astrocytes with radial processes in the dentate gyrus share many of the characteristics of radial glia and appear to act as precursor cells for adult dentate neurogenesis. Using the chemoconvulsant kainate and transgenic mice with human glial-fibrillary acidic protein (hGFAP) promoter-controlled enhanced green fluorescent protein (EGFP) expression, we examined the proliferation, morphology and electrophysiological properties of astrocytes in the neurogenic subgranular zone of the dentate gyrus in control animals and upon the induction of seizure-induced cell proliferation, three days post-kainate. EGFP-positive cells with and without radial processes could easily be distinguished. Kainate treatment caused a significant increase in the total number of proliferating EGFP-positive cells, particularly a tenfold elevation in the number of proliferating radial glia-like astrocytes, and also caused a preferential shift in the dividing cell population towards cells expressing EGFP. Immunohistochemical analysis revealed a surprisingly low proportion of cells coexpressing the astroglial marker S100β and EGFP. Kainate increased the number of EGFP-positive, S100β-positive and S100β-positive–EGFP-positive astrocytes in the subgranular zone. We also report a subset of faintly EGFP-positive cells expressing markers of early neuronal differentiation. Patch-clamp analysis revealed the presence of three functionally different populations of EGFP-positive cells in both kainate and control tissue. We conclude that there is an early increase in proliferating radial glia-like astrocytes in the dentate after kainate-induced seizures, consistent with a recruitment of precursors for seizure-induced neurogenesis.

Ancillary