Role of taurine uptake on the induction of long-term synaptic potentiation

Authors

  • Nuria Del Olmo,

    1. Departamento de Psicobiología, Facultad Psicología, UNED, Madrid, Spain
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    • *

      N.d.O. and L.M.S. contributed equally to this work.

  • Luz M. Suárez,

    1. Servicio de Neurobiología, Departamento de Investigación, Hospital Ramón y Cajal, Ctra. de Colemenar Km 9, 28034 Madrid, Spain
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    • *

      N.d.O. and L.M.S. contributed equally to this work.

  • Luis M. Orensanz,

    1. Servicio de Neurobiología, Departamento de Investigación, Hospital Ramón y Cajal, Ctra. de Colemenar Km 9, 28034 Madrid, Spain
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  • Francisco Suárez,

    1. Servicio de Neurobiología, Departamento de Investigación, Hospital Ramón y Cajal, Ctra. de Colemenar Km 9, 28034 Madrid, Spain
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  • Julián Bustamante,

    1. Departamento de Fisiología, Facultad de Medicina, Universidad Complutense, 28040 Madrid, Spain
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  • José M. Duarte,

    1. Servicio de Neurobiología, Departamento de Investigación, Hospital Ramón y Cajal, Ctra. de Colemenar Km 9, 28034 Madrid, Spain
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  • Rafael Martín Del Río,

    1. Servicio de Neurobiología, Departamento de Investigación, Hospital Ramón y Cajal, Ctra. de Colemenar Km 9, 28034 Madrid, Spain
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  • José M. Solís

    1. Servicio de Neurobiología, Departamento de Investigación, Hospital Ramón y Cajal, Ctra. de Colemenar Km 9, 28034 Madrid, Spain
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: Dr J. M. Solís, as above.
E-mail: jose.m.solis@hrc.es

Abstract

Taurine application in the CA1 area of rat hippocampal slices induces a long-lasting potentiation of excitatory synaptic transmission that has some mechanistic similitude with the late phase of long-term potentiation (L-LTP). Previous indirect evidence such as temperature and sodium dependence indicated that taurine uptake is one of the primary steps leading to the taurine-induced synaptic potentiation. We show that taurine-induced potentiation is not related to the intracellular accumulation of taurine and is not impaired by 2-guanidinoethanesulphonic acid, a taurine transport inhibitor that is a substrate of taurine transporter. We have found that taurine uptake in hippocampal synaptosomes was inhibited by SKF 89976A, a GABA uptake blocker that is not transportable by GABA transporters. SKF 89976A prevents the induction of synaptic potentiation by taurine application. This effect is neither mimicked by nipecotic acid, a broad inhibitor of GABA transporters that does not affect taurine uptake, nor by NO-711, a specific and potent inhibitor of GABA transporter GAT-1. In addition, L-LTP induced by trains of high-frequency stimulation is also inhibited by SKF 89976A, and taurine, at a concentration that does not change basal synaptic transmission, overcomes such inhibition. We conclude that taurine induces synaptic potentiation through the activation of a system transporting taurine and that taurine uptake is required for the induction of synaptic plasticity phenomena such as L-LTP.

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