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Video. S1. Time-lapse laser scanning microscopy of TgN(GFAP-EGFP) transgenic mice reveals two different modes of motility of astroglial processes. 12 optical planes separated by 0.5 ?m are shown over a total time of 25 min (each stack separated by 30 s). Lamellipodial like gliding can be seen in the top right planes (arrowheads), filopodial like protrusions in the planes shown on the lower right (arrow). See also Fig. 2B of the printed paper.

Video. S2. Filopodial like protrusions of astrocytes in acutely isolated brain stem slices. Single planes of the original data set (z-stack) obtained by multi-photon laser scanning recordings are displayed on the left, the corresponding 3D-reconstructions after deconvolution and rendering on the right. z-Stack datasets were recorded every min, the movie shows a total time of 10 min.

Video. S3. Motility of cortical astrocytes revealed by three-dimensional laser scanning time-lapse microscopy. Image stacks (10 ?m deep, z separation 0.5 ?m, 20 sections) were recorded every 60 s for 13 min in an acutely isolated brain slice (cortex) of a postnatal day 4 mouse and displayed as maximum intensity projections. The scale bar represents 10 ?m.

Video. S4. Motility of hippocampal astrocytes revealed by three-dimensional laser scanning time-lapse microscopy. Image stacks (10 ?m deep, z separation 0.5 ?m, 20 sections) were recorded every 60 s for 15 min in an acutely isolated brain slice (hippocampus) of a postnatal day 4 mouse and displayed as maximum intensity projections. The scale bar represents 10 ?m.

FilenameFormatSizeDescription
EJN_3689_sm_suppmovie1.mov1005KSupporting info item
EJN_3689_sm_suppmovie2.mov936KSupporting info item
EJN_3689_sm_suppmovie3.mov405KSupporting info item
EJN_3689_sm_suppmovie4.mov600KSupporting info item

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