Myelination triggers local loss of axonal CNR/protocadherinα family protein expression

Authors

  • Hirofumi Morishita,

    1. KOKORO Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka University, 1–3 Yamadaoka, Suita 565–0871, Japan
    2. Course of Medical Biosignaling, Graduate School of Medicine, Osaka University, Japan
    3. Laboratory of Neurobiology and Behavioural Genetics, National Institute for Physiological Sciences, Japan
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  • Masahumi Kawaguchi,

    1. KOKORO Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka University, 1–3 Yamadaoka, Suita 565–0871, Japan
    2. Course of Medical Biosignaling, Graduate School of Medicine, Osaka University, Japan
    3. Laboratory of Neurobiology and Behavioural Genetics, National Institute for Physiological Sciences, Japan
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  • Yoji Murata,

    1. KOKORO Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka University, 1–3 Yamadaoka, Suita 565–0871, Japan
    2. Laboratory of Neurobiology and Behavioural Genetics, National Institute for Physiological Sciences, Japan
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  • Chika Seiwa,

    1. Department of Neurobiology, Tokyo Metropolitan Institute of Gerontology, Japan
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  • Shun Hamada,

    1. KOKORO Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka University, 1–3 Yamadaoka, Suita 565–0871, Japan
    2. Laboratory of Neurobiology and Behavioural Genetics, National Institute for Physiological Sciences, Japan
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  • Hiroaki Asou,

    1. Department of Neurobiology, Tokyo Metropolitan Institute of Gerontology, Japan
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  • Takeshi Yagi

    1. KOKORO Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka University, 1–3 Yamadaoka, Suita 565–0871, Japan
    2. Course of Medical Biosignaling, Graduate School of Medicine, Osaka University, Japan
    3. Laboratory of Neurobiology and Behavioural Genetics, National Institute for Physiological Sciences, Japan
    4. CREST of Japan Science and Technology Agency, Japan
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Dr Takeshi Yagi, 1KOKORO Biology Group, as above.
E-mail: yagi@fbs.osaka-u.ac.jp

Abstract

The cadherin-related neuronal receptor (CNR)/protocadherin (Pcdh) α family is one of the diverse protocadherin families expressed in developing axons. We observed a strong axonal expression of these proteins at late embryonic and early postnatal stages corresponding to regions where fibers had not yet been myelinated. We therefore followed the postnatal localization of CNR/Pcdhα protein in major axonal tracts, such as the internal capsule, lateral olfactory tract, and optic nerve, and found that its axonal localization was dramatically lost in parallel with the increased expression of myelin markers. Moreover, the hypomyelinated optic nerve tracts of the myelin-deficient Shiverer mouse exhibited elevated levels of CNR/Pcdhα expression. These axonal expression patterns of CNR/Pcdhα in wild-type and Shiverer mice were similar to those of growth associated protein 43 (GAP-43) and L1, both of which are associated with axonal maturation. Thus, myelination may be a trigger for the local loss of axonal CNR/Pcdhα protein, and this process may be important in the maturation of neural circuits.

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