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Fig. S1. Generation of Wlds transgenic rats Southern blot of hemizygous Wlds transgenic rat genomic DNA and wild-type controls with similar gel loading hybridised with the Wlds cDNA. In each case the transgene band (1.1 kb) is substantially stronger than the endogenous band (3.3 kb), which probably represents the endogenous Nmnat-1 gene. Thus, each line has a multi-copy transgene insertion.

Fig. S2. Expression of Wlds protein in rat and mouse Western blot with the Wld-18 antibody showing the expression level of Wlds protein (43 kD) in brain and spinal cord of adult transgenic rat line 79 and Wlds mutant mouse. No difference between the level of expression of Wlds protein in the transgenic rat and Wlds mouse can be seen. However, there is a higher level of Wlds protein in brain than in spinal cord in both species.

Fig. S3. Investigation of the Wlds protective mechanism One recent proposal that the inflammatory mediator CD200 could mediate the Wlds phenotype because it is more abundant in Wlds mice than in wild-type, was tested (Chitnis et al., Eur. Charcot Foundation Symp. December 2003). Western blot showing no change in levels of CD200 protein in the spinal cord of Wlds transgenic rats in comparison to wild-type controls at 4 months of age.

FilenameFormatSizeDescription
EJN_3833_sm_FigS1.tif299KSupporting info item
EJN_3833_sm_FigS2.tif404KSupporting info item
EJN_3833_sm_FigS3.tif354KSupporting info item

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