Get access

A synthetic NCAM-derived peptide, FGL, protects hippocampal neurons from ischemic insult both in vitro and in vivo

Authors

  • Galina G. Skibo,

    1. Protein Laboratory, Institute of Molecular Pathology, Panum Institute, University of Copenhagen, Blegdamsvej 3C, bld. 6.2, DK-2200 Copenhagen N, Denmark
    2. Department of Cytology, Bogomoletz Institute of Physiology, Bogomoletz str., 4, Kiev 01024, Ukraine
    Search for more papers by this author
  • Iryna V. Lushnikova,

    1. Protein Laboratory, Institute of Molecular Pathology, Panum Institute, University of Copenhagen, Blegdamsvej 3C, bld. 6.2, DK-2200 Copenhagen N, Denmark
    2. Department of Cytology, Bogomoletz Institute of Physiology, Bogomoletz str., 4, Kiev 01024, Ukraine
    Search for more papers by this author
  • Kyrill Y. Voronin,

    1. Protein Laboratory, Institute of Molecular Pathology, Panum Institute, University of Copenhagen, Blegdamsvej 3C, bld. 6.2, DK-2200 Copenhagen N, Denmark
    2. Department of Cytology, Bogomoletz Institute of Physiology, Bogomoletz str., 4, Kiev 01024, Ukraine
    Search for more papers by this author
  • Oksana Dmitrieva,

    1. Protein Laboratory, Institute of Molecular Pathology, Panum Institute, University of Copenhagen, Blegdamsvej 3C, bld. 6.2, DK-2200 Copenhagen N, Denmark
    Search for more papers by this author
  • Tatiana Novikova,

    1. Protein Laboratory, Institute of Molecular Pathology, Panum Institute, University of Copenhagen, Blegdamsvej 3C, bld. 6.2, DK-2200 Copenhagen N, Denmark
    2. ENKAM Pharmaceuticals A/S, Fruebjergvej 3, DK-2100 Copenhagen Ø, Denmark
    Search for more papers by this author
  • Boris Klementiev,

    1. Protein Laboratory, Institute of Molecular Pathology, Panum Institute, University of Copenhagen, Blegdamsvej 3C, bld. 6.2, DK-2200 Copenhagen N, Denmark
    2. ENKAM Pharmaceuticals A/S, Fruebjergvej 3, DK-2100 Copenhagen Ø, Denmark
    Search for more papers by this author
  • Elisabetta Vaudano,

    1. ENKAM Pharmaceuticals A/S, Fruebjergvej 3, DK-2100 Copenhagen Ø, Denmark
    Search for more papers by this author
  • Vladimir A. Berezin,

    1. Protein Laboratory, Institute of Molecular Pathology, Panum Institute, University of Copenhagen, Blegdamsvej 3C, bld. 6.2, DK-2200 Copenhagen N, Denmark
    Search for more papers by this author
  • Elisabeth Bock

    1. Protein Laboratory, Institute of Molecular Pathology, Panum Institute, University of Copenhagen, Blegdamsvej 3C, bld. 6.2, DK-2200 Copenhagen N, Denmark
    Search for more papers by this author

Dr E. Bock, as above.
E-mail: Bock@plab.ku.dk

Abstract

There is a major unmet need for development of innovative strategies for neuroprotection against ischemic brain injury. Here we show that FGL, a neural cell adhesion molecule (NCAM)-derived peptide binding to and inducing phosphorylation of the fibroblast growth factor receptor (FGFR), acts neuroprotectively after an ischemic insult both in vitro and in vivo. The neuroprotective activity of FGL was tested in vitro on dissociated rat hippocampal neurons and hippocampal slice cultures, using a protocol of oxygen–glucose deprivation (OGD). FGL protected hippocampal neurons from damage and maintained or restored their metabolic and presynaptic activity, both if employed as a pretreatment alone to OGD, and if only applied after the insult. In vivo 24 h pretreatment with a single suboccipital injection of FGL significantly protected hippocampal CA1 neurons from death in a transient global ischemia model in the gerbil. We conclude that FGL promotes neuronal survival after ischemic brain injury.

Get access to the full text of this article

Ancillary