R.M.-I and M.J. contributed equally to this study.
Vesicular glutamate transporter 3 (VGLUT3) identifies spatially segregated excitatory terminals in the rat substantia nigra
Version of Record online: 8 FEB 2006
European Journal of Neuroscience
Volume 23, Issue 4, pages 1063–1070, February 2006
How to Cite
Martín-Ibañez, R., Jenstad, M., Berghuis, P., Edwards, R. H., Hioki, H., Kaneko, T., Mulder, J., Canals, J. M., Ernfors, P., Chaudhry, F. A. and Harkany, T. (2006), Vesicular glutamate transporter 3 (VGLUT3) identifies spatially segregated excitatory terminals in the rat substantia nigra. European Journal of Neuroscience, 23: 1063–1070. doi: 10.1111/j.1460-9568.2006.04601.x
- Issue online: 8 FEB 2006
- Version of Record online: 8 FEB 2006
- Received 14 August 2004, revised 14 November 2005, accepted 7 December 2005
- basal ganglia;
The excitability of dopaminergic (DA) neurons in the substantia nigra is controlled by the convergent activity of multiple glutamatergic afferents. Here, we show that vesicular glutamate transporter 3 (VGLUT3)-immunoreactive (ir) terminals segregate to the perisomatic region of DA neurons in the substantia nigra pars compacta, and VGLUT3 decorates a synapse population distinct from those marked by vesicular glutamate transporters 1 and 2. VGLUT3-ir nerve endings form asymmetric terminals on DA neurons. Retrograde tracing suggests the superior colliculus as an origin of excitatory VGLUT3-ir afferents. Collectively, our data indicate that VGLUT3 identifies a novel excitatory terminal subset that contributes to the tuning of DA cell excitability in the substantia nigra.