*Present address: Department of Pharmacology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814–4799, USA.
Calcium-triggered exit of F-actin and IP3 3-kinase A from dendritic spines is rapid and reversible
Article first published online: 13 NOV 2006
European Journal of Neuroscience
Volume 24, Issue 9, pages 2491–2503, November 2006
How to Cite
Schell, M. J. and Irvine, R. F. (2006), Calcium-triggered exit of F-actin and IP3 3-kinase A from dendritic spines is rapid and reversible. European Journal of Neuroscience, 24: 2491–2503. doi: 10.1111/j.1460-9568.2006.05125.x
- Issue published online: 13 NOV 2006
- Article first published online: 13 NOV 2006
- Received 20 April 2006, revised 2 August 2006, accepted 19 August 2006
Supplementary Fig. 1. IP3K and F-actin move away from presynaptic sites when cultures are stimulated with glutamate. (A) Unstimulated neuron stained with IP3K (red) and the presynaptic marker synapsin (green). The majority of IP3K staining appears postsynaptic, adjacent to synapsin-positive presynaptic terminals. (B) 10 seconds after stimulation with 100 KML-glu/10 KMD-ser, IP3K staining has moved away from apposition to presynaptic sites and assumes a striated pattern in the dendritic shafts. IP3K staining (C) is coincident with phalloidin (E, F) before (G) and after (H) stimulation for 2 min. IP3K (I, J) staining is distinct from the microtubule marker MAP2 (K, L) before (M) and 2 min after (L) glutamate stimulation.
Supplementary movie 1. Time-lapse movie of GFP-IP3K relocalization following the stimulation of glutamate receptors. L-glu/D-ser were added immediately before collection of the first frame.
Supplementary movie 2. Time-lapse movie of GFP-IP3K returning to spines following the removal of the stimulus and addition of 10 μM MK-801.
Supplementary movie 3. 3-dimensional rendering of the relationship between IP3K (green) and the ER (red) in hippocampal neurons grown in culture for 25 days.
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