Supplementary Fig. 1. IP3K and F-actin move away from presynaptic sites when cultures are stimulated with glutamate. (A) Unstimulated neuron stained with IP3K (red) and the presynaptic marker synapsin (green). The majority of IP3K staining appears postsynaptic, adjacent to synapsin-positive presynaptic terminals. (B) 10 seconds after stimulation with 100 KML-glu/10 KMD-ser, IP3K staining has moved away from apposition to presynaptic sites and assumes a striated pattern in the dendritic shafts. IP3K staining (C) is coincident with phalloidin (E, F) before (G) and after (H) stimulation for 2 min. IP3K (I, J) staining is distinct from the microtubule marker MAP2 (K, L) before (M) and 2 min after (L) glutamate stimulation.

Supplementary movie 1. Time-lapse movie of GFP-IP3K relocalization following the stimulation of glutamate receptors. L-glu/D-ser were added immediately before collection of the first frame.

Supplementary movie 2. Time-lapse movie of GFP-IP3K returning to spines following the removal of the stimulus and addition of 10 μM MK-801.

Supplementary movie 3. 3-dimensional rendering of the relationship between IP3K (green) and the ER (red) in hippocampal neurons grown in culture for 25 days.

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