A.S.C., A.R.O.F., M.H.L. and G.N.M.H. contributed equally to this work.
The interaction between prion protein and laminin modulates memory consolidation
Version of Record online: 11 DEC 2006
European Journal of Neuroscience
Volume 24, Issue 11, pages 3255–3264, December 2006
How to Cite
Coitinho, A. S., Freitas, A. R. O., Lopes, M. H., Hajj, G. N. M., Roesler, R., Walz, R., Rossato, J. I., Cammarota, M., Izquierdo, I., Martins, V. R. and Brentani, R. R. (2006), The interaction between prion protein and laminin modulates memory consolidation. European Journal of Neuroscience, 24: 3255–3264. doi: 10.1111/j.1460-9568.2006.05156.x
- Issue online: 11 DEC 2006
- Version of Record online: 11 DEC 2006
- Received 31 May 2006, revised 10 August 2006, accepted 4 September 2006
- cellular prion;
Cellular prion protein (PrPc) has a pivotal role in prion diseases. PrPc is a specific receptor for laminin (LN) γ1 peptide and several lines of evidence indicate that it is also involved in neural plasticity. Here we investigated whether the interaction between PrPc and LN plays a role in rat memory formation. We found that post-training intrahippocampal infusion of PrPc-derived peptides that contain the LN binding site ( and ) or of anti-PrPc or anti-LN antibodies that inhibit PrPc–LN interaction impaired inhibitory avoidance memory retention. The amnesic effect of anti-PrPc antibodies and peptide was reversed by co-infusion of a LN γ1 chain-derived peptide containing the PrPc-binding site, suggesting that PrPc–LN interaction is indeed crucial for memory consolidation. In addition, peptide and anti-PrPc or anti-LN antibodies also inhibited the activation of hippocampal cAMP-dependent protein kinase A (PKA) and extracellular regulated kinase (ERK1/2), two kinases that mediate the up-regulation of signaling pathways needed for consolidation of inhibitory avoidance memory. Our findings show that, through its interaction with LN, hippocampal PrPc plays a critical role in memory processing and suggest that this role is mediated by activation of both PKA and ERK1/2 signaling pathways.