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Figure S1. Marker molecules exhibit increased phosphorylation levels one hour after LTP induction in CA1 in vivo. Quantification of immunoblot signals from lysates (20 μg protein/lane) of tetanised and control CA1 tissue of male BL6 WT mice harvested one hour (1h) after LTP induction. Immunoblots were probed with antibodies against (A) phospho-Ser603-synapsin I (171%, n = 4, F1-6 = 8.27, *P < 0.05), (B) phospho-Thr286-αCaMKII (150%, n = 5, F1-8 = 13.5, *P < 0.05) and (C) phospho-Ser129/133-CREB (172%, n = 4, F1-6 = 8.58, *P < 0.05). Figure S2. Marker molecules exhibit increased phosphorylation levels one hour after LTP induction in DG in vivo. Quantification of immunoblot signals from lysates (20 μg protein/lane) of tetanised and control DG tissue of BL6 WT mice harvested one hour (1h) after LTP induction. Immunoblots were probed with antibodies against (A) phospho-Ser603-synapsin I (122%, n = 4, F1-6 = 9.63, *P < 0.05), (B) phospho-Thr286-αCaMKII (164%, n = 8; F1-14 = 792; ***P < 0.001) and (C) phospho-Ser133-CREB (151%, n = 6, F1-10 = 26.8, ***P < 0.001). Figure S3. GSK-3 expression in tet/GSK-3 mice. Immunoblot of brain from 15 week tet/GSK-3β mice. GSK-3α and endogenous GSK-3β levels are unchanged but transgenic myc-tagged GSK-3β, identifiable as a slower migrating band, is increased.

FilenameFormatSizeDescription
EJN_5245_sm_FigS1.tif54KSupporting info item
EJN_5245_sm_FigS2.tif54KSupporting info item
EJN_5245_sm_FigS3.tif69KSupporting info item

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