Appendix S1. Supplementary Results. Sensitivity to parameter values. Video S1. NOS in subpopulations of Kenyon cell bodies and in the calyx. The video begins at the frontal brain surface and shows an interpolated stack of frontal sections; medial is left. The tens of thousand tiny cell bodies atop and around the calyx neuropil belong to the Kenyon cells. The NOS-expressing subpopulations occupy several distinct regions, separated by regions of Kenyon cells that do not express NOS. Comparatively weakly NOS-positive axons stream from the cell bodies to the top of the stalk. The expression of NOS increases dramatically in the axons as they enter the stalk. The diffuse staining in the primary calyx (large kidney-shaped region visible during the first two thirds of the video) is caused by NOS-expression in Kenyon cell dendrites (the fine, sharply stained, beaded fibres are of extrinsic origin). The accessory calyx neuropil, which comes into view on the right about two-thirds into the video, is virtually unstained. The scattered fine, sharply stained, beaded fibres in the calyx are of extrinsic origin. Heavily stained axons that stream down along the medial (left) surface of the brain belong to small ocellar interneurones (Kurylas et al., 2005). Video S2. NO-sensitive Kenyon cell axons in the tube cores of the lobes. NO-induced cGMP immunofluorescence, 3D confocal reconstruction from a 150 μm frontal brain slice. The far surface of the slice passes through the lower end of the stalk, where the Kenyon cell axons bifurcate to enter the α-lobe (upper half of the frame) and β-lobe (lower half of the frame). The conspicuous "hay-fork" structure in the upper half of the frame represents cGMP-immunopositive bundles of Kenyon cell axons residing in the parallel cores of the tubelets of the α-lobe.

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