Present address: Center for Neurologic Diseases, Dept. of Neurology, Harvard Medical School, Boston, MA 02115, USA.
A brief, but repeated, swimming protocol is sufficient to overcome amyloid β-protein inhibition of hippocampal long-term potentiation
Article first published online: 29 AUG 2007
European Journal of Neuroscience
Volume 26, Issue 5, pages 1289–1298, September 2007
How to Cite
Li, S., Feig, L. A. and Hartley, D. M. (2007), A brief, but repeated, swimming protocol is sufficient to overcome amyloid β-protein inhibition of hippocampal long-term potentiation. European Journal of Neuroscience, 26: 1289–1298. doi: 10.1111/j.1460-9568.2007.05760.x
- Issue published online: 29 AUG 2007
- Article first published online: 29 AUG 2007
- Received 26 February 2007, revised 2 July 2007, accepted 11 July 2007
- amyloid β-protein;
Alzheimer's disease starts as an almost imperceptible malady, first observed clinically as a mild memory problem. Accumulating genetic and biochemical data have suggested that amyloid β-protein (Aβ) plays an important role in this memory loss, and Aβ has been shown to suppress long-term potentiation (LTP), a cellular model for memory and learning. Here we show that a very brief (3 min) swimming, twice daily for 2 weeks, rescues LTP inhibition in the CA1 region of hippocampal slices caused by Aβ42 or Aβ40 carrying the Arctic mutation using a theta burst stimulation (TBS) protocol. Whereas the input–output curve was not affected, the paired-pulse ratio was reduced in mice receiving our repeated swimming protocol, suggesting a possible involvement of presynaptic facilitation. Similar to swimming, Aβ's inhibition of LTP could be rescued with the adenylyl cyclase, forskolin. Interestingly, this swimming protocol produced conditions in which a weak-TBS could invoke LTP not observed in naïve mice, which again was mimicked by forskolin. In contrast, the protein kinase A (PKA) inhibitor, H89, blocked both the forskolin and swimming potentiation of LTP; these data implicate cAMP/PKA signaling in the protective effect of swimming and mediating Aβ′ detrimental effects. Our data add a new simple behavior paradigm that shows the importance of an environmental factor in reversing the pathophysiological effects of Aβ, and suggest new therapeutic avenues.