T.N. and A.T. contributed equally to this work.
Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice
Version of Record online: 17 APR 2009
© The Authors (2009). Journal Compilation © Federation of European Neuroscience Societies and Blackwell Publishing Ltd
European Journal of Neuroscience
Special Issue: SPECIAL FEATURE: THE FUNCTIONS OF SLEEP
Volume 29, Issue 9, pages 1842–1852, May 2009
How to Cite
Nakagomi, T., Taguchi, A., Fujimori, Y., Saino, O., Nakano-Doi, A., Kubo, S., Gotoh, A., Soma, T., Yoshikawa, H., Nishizaki, T., Nakagomi, N., Stern, D. M. and Matsuyama, T. (2009), Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice. European Journal of Neuroscience, 29: 1842–1852. doi: 10.1111/j.1460-9568.2009.06732.x
- Issue online: 5 MAY 2009
- Version of Record online: 17 APR 2009
- Received 15 October 2008, revised 18 February 2009, accepted 25 February 2009
- cortical infarction;
The CNS has the potential to marshal strong reparative mechanisms, including activation of endogenous neurogenesis, after a brain injury such as stroke. However, the response of neural stem/progenitor cells to stroke is poorly understood. Recently, neural stem/progenitor cells have been identified in the cerebral cortex, as well as previously recognized regions such as the subventricular or subgranular zones of the hippocampus, suggesting that a contribution of cortex-derived neural stem/progenitor cells may repair ischemic lesions of the cerebral cortex. In the present study, using a highly reproducible murine model of cortical infarction, we have found nestin-positive cells in the post-stroke cerebral cortex, but not in the non-ischemic cortex. Cells obtained from the ischemic core of the post-stroke cerebral cortex formed neurosphere-like cell clusters expressing nestin; such cells had the capacity for self-renewal and differentiated into electrophysiologically functional neurons, astrocytes and myelin-producing oligodendrocytes. Nestin-positive cells from the stroke-affected cortex migrated into the peri-infarct area and differentiated into glial cells in vivo. Although we could not detect differentiation of nestin-positive cells into neurons in vivo, our current observations indicate that endogenous neural stem/progenitors with the potential to become neurons can develop within post-stroke cerebral cortex.