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Brain tissue oxygen amperometry in behaving rats demonstrates functional dissociation of dorsal and ventral hippocampus during spatial processing and anxiety
Version of Record online: 25 NOV 2010
© 2010 The Authors. European Journal of Neuroscience © 2010 Federation of European Neuroscience Societies and Blackwell Publishing Ltd
European Journal of Neuroscience
Volume 33, Issue 2, pages 322–337, January 2011
How to Cite
McHugh, S. B., Fillenz, M., Lowry, J. P., Rawlins, J. N. P. and Bannerman, D. M. (2011), Brain tissue oxygen amperometry in behaving rats demonstrates functional dissociation of dorsal and ventral hippocampus during spatial processing and anxiety. European Journal of Neuroscience, 33: 322–337. doi: 10.1111/j.1460-9568.2010.07497.x
- Issue online: 17 JAN 2011
- Version of Record online: 25 NOV 2010
- Received 7 June 2010, revised 13 September 2010, accepted 7 October 2010
- constant potential amperometry;
- dorsal hippocampus;
- tissue oxygen voltammetry;
- ventral hippocampus
Traditionally, the function of the hippocampus (HPC) has been viewed in unitary terms, but there is growing evidence that the HPC is functionally differentiated along its septotemporal axis. Lesion studies in rodents and functional brain imaging in humans suggest a preferential role for the septal HPC in spatial learning and a preferential role for the temporal HPC in anxiety. To better enable cross-species comparison, we present an in vivo amperometric technique that measures changes in brain tissue oxygen at high temporal resolution in freely-moving rats. We recorded simultaneously from the dorsal (septal; dHPC) and ventral (temporal; vHPC) HPC during two anxiety tasks and two spatial tasks on the radial maze. We found a double-dissociation of function in the HPC, with increased vHPC signals during anxiety and increased dHPC signals during spatial processing. In addition, dHPC signals were modulated by spatial memory demands. These results add a new dimension to the growing consensus for a differentiation of HPC function, and highlight tissue oxygen amperometry as a valuable tool to aid translation between animal and human research.