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Fig. S1. Comparison of immunohistochemical staining pattern for ,mu-,opioid receptor (MOR) in the neostriatum in serial sections. Section shown in a and b were stained with anti-MOR antibody raised by guinea pig raised against C-terminal 30 amino acids (residues 369-398; Kaneko et al., 1995) and 15 amino acids (residues 384-398; Chemicon).

Fig. S2. Cyto- and chemoarchitecture of the substantia nigra. Two adjacent series of parasagittal sections from control rat mesencephalon were immunostained for tyrosine hydroxylase (TH;a, b, c) and stained for Nissl (a′, b′, c′). Although the TH-immunopositive dendrites were scattered in the SNr (encircled with white broken circles), all the TH-positive cell bodies and bushy arborizations of dense TH-positive dendrites were restricted to the SNc. For TH immunocytochemistry, the sections were incubated serially with 1/1000-diluted avidin-biotinylated peroxidase complex (ABC-Elite). The bound peroxidase was visualized by the reaction with diaminobenzidine and H2O2. SNc-CC, cell cluster region of the ventral tier of the SNc.

Table S1. Comparison between palGFP- and GFP-labeled axon varicosities.

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