Present address: Department of Physiology and Pharmacology, Oregon Health & Science University, Portland, OR 97239, USA.
Activation of the mitochondrial permeability transition pore modulates Ca2+ responses to physiological stimuli in adult neurons
Article first published online: 24 JAN 2011
© 2011 The Authors. European Journal of Neuroscience © 2011 Federation of European Neuroscience Societies and Blackwell Publishing Ltd
European Journal of Neuroscience
Volume 33, Issue 5, pages 831–842, March 2011
How to Cite
Barsukova, A., Komarov, A., Hajnóczky, G., Bernardi, P., Bourdette, D. and Forte, M. (2011), Activation of the mitochondrial permeability transition pore modulates Ca2+ responses to physiological stimuli in adult neurons. European Journal of Neuroscience, 33: 831–842. doi: 10.1111/j.1460-9568.2010.07576.x
- Issue published online: 8 MAR 2011
- Article first published online: 24 JAN 2011
- Received 31 August 2010, revised 18 October 2010, accepted 1 December 2010
Fig. S1. Representative traces of mitochondrial Ca2+ responses following stimulation of adult WT and CyPD-KO cortical neurons with DHPG, and quantification of mitochondrial Ca2+ levels prior to stimulation and at peak in response to DHPG.
Fig. S2. Pretreatment with the mitochondrial uncoupler FCCP (450 nM) for 3 min abolishes mitochondrial Ca2+ uptake following perfusion with ATP in neurons prepared from WT and CyPD-KO, absence of mitochondrial Ca2+ responses following depolarization in the absence of extracellular Ca2+, and pretreatment with mitochondrial uncoupler FCCP (450 nM) for 3 min abolishes mitochondrial Ca2+ uptake in neurons prepared from either genotype following depolarization with KCl.
Fig. S3. Mitochondrial morphology in neurons remains unaltered following perfusion with ATP and KCl.
Fig. S4. Mitochondrial Ca2+ responses following simultaneous stimulation with either ATP or depolarization in WT and CyPD-KO adult cortical neurons treated with CsA.
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