Riluzole is a potent drug to protect neonatal rat hypoglossal motoneurons in vitro from excitotoxicity due to glutamate uptake block
Version of Record online: 17 FEB 2011
© 2011 The Authors. European Journal of Neuroscience © 2011 Federation of European Neuroscience Societies and Blackwell Publishing Ltd
European Journal of Neuroscience
Volume 33, Issue 5, pages 899–913, March 2011
How to Cite
Cifra, A., Nani, F. and Nistri, A. (2011), Riluzole is a potent drug to protect neonatal rat hypoglossal motoneurons in vitro from excitotoxicity due to glutamate uptake block. European Journal of Neuroscience, 33: 899–913. doi: 10.1111/j.1460-9568.2010.07579.x
- Issue online: 8 MAR 2011
- Version of Record online: 17 FEB 2011
- Received 23 June 2010, revised 30 November 2010, accepted 1 December 2010
Fig. S1. Astrocyte population in the P4 rat nucleus hypoglossus and spinal cord. (A) Example of a confocal image (20×) of hypoglossal nuclei labeled with GFAP (fibrous astrocyte marker; green) and Hoechst 33342 (blue). The inset (higher magnification) shows how GFAP labeling is limited to the central canal profile. (B) Example of GFAP (green) and S100B (protoplasmic astrocytic marker; red) immunoreactivity of the spinal cord ventral horn (fluorescence microscopy image, l0×). (C) Histological example of GFAP (green) and DAPI (blue) staining of cell in the rat spinal cord (fluorescence microscopy image, 5×).
Fig. S2. Immunostaining of motoneurons and astrocytes in the nucleus hypoglossus of P4 rat. (A) Example of a confocal images (l0×) of hypoglossal nuclei labeled with SMI 32 (motoneuron marker; green) and Hoechst 33342 (blue). Inset shows schematic location of nuclei in brainstem section. (B) Higher magnification (20×) of the example in A, showing the region of interest (ROI: white box; 100 000 µm2) used for cell counting. (C) Histological example of S100B (green; astrocytes) and Hoechst 33342 (blue) staining.
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