C.E.P.-L. and T.M.S. contributed equally to this study.
Intrinsic phototransduction persists in melanopsin-expressing ganglion cells lacking diacylglycerol-sensitive TRPC subunits
Article first published online: 24 JAN 2011
European Journal of Neuroscience © 2011 Federation of European Neuroscience Societies and Blackwell Publishing Ltd. No claim to original US government works
European Journal of Neuroscience
Volume 33, Issue 5, pages 856–867, March 2011
How to Cite
Perez-Leighton, C. E., Schmidt, T. M., Abramowitz, J., Birnbaumer, L. and Kofuji, P. (2011), Intrinsic phototransduction persists in melanopsin-expressing ganglion cells lacking diacylglycerol-sensitive TRPC subunits. European Journal of Neuroscience, 33: 856–867. doi: 10.1111/j.1460-9568.2010.07583.x
- Issue published online: 8 MAR 2011
- Article first published online: 24 JAN 2011
- Received 20 August 2010, revised 29 November 2010, accepted 3 December 2010
Fig. S1. Specificity of anti-melanopsin antibody. Confocal images of Opn4−/− (A) and WT (B) adult retinas imaged at the GCL. Retinas were immunostained and imaged for melanopsin using the anti-melanopsin antibody under identical conditions. Notice the staining of ganglion cells in the WT retina (B) and lack of staining in the Opn4−/− retina (A). Scale bar: 100 μm.
Fig. S2. Real-time PCR analysis in P6–P8 retinas. mRNA expression levels of TRPC3, TRPC6 and TRPC7 subunits were analysed in P6–P8 WT retinas. mRNA expression levels for TRPC transcripts are normalized to β-actin. One-way anova indicated significant differences between TRPC subunits expression (F3,14 = 227.5, P = 0.0001). A Tukey HSD showed significant differences between TRPC3 and TRPC6 (q = 17.09, P < 0.0001) and TRPC7 (q = 24.16, P = 0.0001), and no differences between TRPC6 and TRPC7 (q = 3.56, P = 0.065). Data are presented as mean ± SEM. n = 4 retinas per group.
Fig. S3. Immunohistochemical analysis of TRPC−/− adult retinas. Analysis of different cell types in adult retinas revealed no qualitative differences between genotypes. The cells types analysed were: (A) Muller cells, anti-glutamine synthetase antibody; (B) rod bipolar cells, anti-PKCα; (C) cholinergic amacrine cells, anti-ChAT antibody; (D) dopaminergic amacrine cells, anti-tyrosine hydroxylase antibody (see Materials and methods for additional antibody information). Scale bar: 20 μm.
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