Fig. S1. Specificity of anti-melanopsin antibody. Confocal images of Opn4−/− (A) and WT (B) adult retinas imaged at the GCL. Retinas were immunostained and imaged for melanopsin using the anti-melanopsin antibody under identical conditions. Notice the staining of ganglion cells in the WT retina (B) and lack of staining in the Opn4−/− retina (A). Scale bar: 100 μm.

Fig. S2. Real-time PCR analysis in P6–P8 retinas. mRNA expression levels of TRPC3, TRPC6 and TRPC7 subunits were analysed in P6–P8 WT retinas. mRNA expression levels for TRPC transcripts are normalized to β-actin. One-way anova indicated significant differences between TRPC subunits expression (F3,14 = 227.5, = 0.0001). A Tukey HSD showed significant differences between TRPC3 and TRPC6 (= 17.09, < 0.0001) and TRPC7 (= 24.16, = 0.0001), and no differences between TRPC6 and TRPC7 (= 3.56, = 0.065). Data are presented as mean ± SEM. = 4 retinas per group.

Fig. S3. Immunohistochemical analysis of TRPC−/− adult retinas. Analysis of different cell types in adult retinas revealed no qualitative differences between genotypes. The cells types analysed were: (A) Muller cells, anti-glutamine synthetase antibody; (B) rod bipolar cells, anti-PKCα; (C) cholinergic amacrine cells, anti-ChAT antibody; (D) dopaminergic amacrine cells, anti-tyrosine hydroxylase antibody (see Materials and methods for additional antibody information). Scale bar: 20 μm.

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