Present address: Department of Pharmacology and Therapeutics, McGill University, 3649 Promenade Sir-William-Osler, Montreal, Quebec, Canada. H3G 0B1
Functional evidence for D-serine inhibition of non-N-methyl-D-aspartate ionotropic glutamate receptors in retinal neurons
Article first published online: 1 DEC 2011
© 2011 The Authors. European Journal of Neuroscience © 2011 Federation of European Neuroscience Societies and Blackwell Publishing Ltd
European Journal of Neuroscience
Volume 35, Issue 1, pages 56–65, January 2012
How to Cite
Daniels, B. A., Wood, L., Tremblay, F. and Baldridge, W. H. (2012), Functional evidence for D-serine inhibition of non-N-methyl-D-aspartate ionotropic glutamate receptors in retinal neurons. European Journal of Neuroscience, 35: 56–65. doi: 10.1111/j.1460-9568.2011.07925.x
- Issue published online: 2 JAN 2012
- Article first published online: 1 DEC 2011
- Received 13 April 2011, revised 15 September 2011, accepted 27 September 2011
Fig. S1. Control experiments for the effect of exogenously applied DAAO on the kainate-induced calcium response. (A) Mean + SD data from four separate sets of experiments showing the percent increase in the kainate-induced calcium response (relative to the initial response) from cells that showed a > 10% increase in the response following application of 0.25 mg/mL DAAO alone (first bar, n = 7, from 58 cells), when all solutions contained ≥ 4000 units/mL catalase (second bar, n = 3, from 60 cells) or 100 μM edaravone (third bar, n = 3, from 47 cells) and after heat inactivation (fourth bar, n = 3, from all 57 cells). (B) Percentage of cells that showed a > 10% increase in the kainate-induced calcium response following each of the four DAAO application protocols.
Fig.S2. Light-evoked MEA recordings and the effect of NBQX. (A) Cumulative frequency histograms (10 ms bins for 60 sweeps) from the three cells during the same MEA recording demonstrate the three basic response profiles from the spiking neurons to a 500 ms flash of light (green bar). The top trace shows an ON cell, the middle an OFF cell and the bottom an ON–OFF cell. ON and OFF responses were analyzed separately regardless of whether the response came from a pure ON or OFF cell or an ON–OFF cell. (B) Experiments were performed in the standard inhibitory cocktail (strychnine/picrotoxin/MK-801) and the bath application of 20 μm NBQX (2 min) significantly reduced light-evoked spiking. Some recovery was achieved after a washout period of 10–60 min (n = 4 from 183 ON responding cells and 182 OFF responding cells). Data expressed as mean + SD and analyzed with repeated-measures Tukey’s one-way ANOVA**.
As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset by Wiley-Blackwell. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.
|EJN_7925_sm_FigS1.tif||304K||Supporting info item|
|EJN_7925_sm_FigS2.tif||389K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.