Present address: CNRS UMR 7224, Physiopathologie des Maladies du Système Nerveux Central, Paris, France; INSERM U952, Paris, France; UPMC, University Paris 06, Paris, France.
Network activity and spike discharge oscillations in cortical slice cultures from neonatal rat
Article first published online: 25 JAN 2012
© 2012 The Authors. European Journal of Neuroscience © 2012 Federation of European Neuroscience Societies and Blackwell Publishing Ltd
European Journal of Neuroscience
Volume 35, Issue 3, pages 375–388, February 2012
How to Cite
Czarnecki, A., Tscherter, A. and Streit, J. (2012), Network activity and spike discharge oscillations in cortical slice cultures from neonatal rat. European Journal of Neuroscience, 35: 375–388. doi: 10.1111/j.1460-9568.2011.07966.x
- Issue published online: 31 JAN 2012
- Article first published online: 25 JAN 2012
- Received 13 July 2011, revised 14 November 2011, accepted 17 November 2011
- cortical interneurons;
- fast-spiking neurons;
- neuronal avalanches;
- regular-spiking neurons
Network bursts and oscillations are forms of spontaneous activity in cortical circuits that have been described in vivo and in vitro. Searching for mechanisms involved in their generation, we investigated the collective network activity and spike discharge oscillations in cortical slice cultures of neonatal rats, combining multielectrode arrays with patch clamp recordings from individual neurons. The majority of these cultures showed spontaneous collective network activity [population bursts (PBs)] that could be described as neuronal avalanches. The largest of these PBs were followed by fast spike discharge oscillations in the beta to theta range, and sometimes additional repetitive PBs, together forming seizure-like episodes. During such episodes, all neurons showed sustained depolarization with increased spike rates. However, whereas regular-spiking (RS) and fast-spiking (FS) neurons fired during the PBs, only the FS neurons fired during the fast oscillations. Blockade of N-methyl-d-aspartate receptors reduced the depolarization and suppressed both the increased FS neuron firing and the oscillations. To investigate the generation of PBs, we studied the network responses to electrical stimulation. For most of the stimulation sites, the relationship between the stimulated inputs and the evoked PBs was linear. From a few stimulation sites, however, large PBs could be evoked with small inputs, indicating the activation of hub circuits. Taken together, our findings suggests that the oscillations originate from recurrent inhibition in local networks of depolarized inhibitory FS interneurons, whereas the PBs originate from recurrent excitation in networks of RS and FS neurons that is initiated in hub circuits.