Fig. S1. Blood CsA levels measured in experimental animals at time of sacrifice, and in sentinel animals throughout the study duration. CsA levels peak around P50 and decline to levels that have been reported by others (Ubhi et al., 1988) to be sub-clinically efficient by P90.

Fig. S2. The majority of the SC121 positive cells that compose neurospheres co-express Nestin (A–C), GFAPdelta (D–F) while only a few cells express doublecortin (G–I). Bar size is 50 μm in all images (bar in C applies to A–F; bar in I applies to G–I).

Fig. S3. HuCNS-SC phagocytose ROS in vitro. (A) The vast majority of HuCNS-SC express the αvβ5 integrin. (B) Representative images of ARPE19 (top panels) and HuCNS-SC (bottom panels) containing internalized alexa488-labeled ROS. In the presence of antibodies against αvβ5, the amount of internalized ROS is decreased. Inserts show high-magnification merged images of phase contrast and Alexa488 fluorescence, highlighting internalized ROS. (C) Quantification of internalized fluorescence by pixel analysis for HuCNS-SC and ARPE-19, in the presence of anti-αvβ5 antibody or isotype matched control antibody (IgG); ROS, rod outer segment. Bars represent standard error of the mean.

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