T.J.M., B.C., R.D.L. and A.C. contributed equally to this work.
Transplantation of human central nervous system stem cells – neuroprotection in retinal degeneration
Article first published online: 25 JAN 2012
© 2012 The Authors. European Journal of Neuroscience © 2012 Federation of European Neuroscience Societies and Blackwell Publishing Ltd
European Journal of Neuroscience
Volume 35, Issue 3, pages 468–477, February 2012
How to Cite
McGill, T. J., Cottam, B., Lu, B., Wang, S., Girman, S., Tian, C., Huhn, S. L., Lund, R. D. and Capela, A. (2012), Transplantation of human central nervous system stem cells – neuroprotection in retinal degeneration. European Journal of Neuroscience, 35: 468–477. doi: 10.1111/j.1460-9568.2011.07970.x
- Issue published online: 31 JAN 2012
- Article first published online: 25 JAN 2012
- Received 13 October 2011, revised 18 November 2011, accepted 21 November 2011
Fig. S1. Blood CsA levels measured in experimental animals at time of sacrifice, and in sentinel animals throughout the study duration. CsA levels peak around P50 and decline to levels that have been reported by others (Ubhi et al., 1988) to be sub-clinically efficient by P90.
Fig. S2. The majority of the SC121 positive cells that compose neurospheres co-express Nestin (A–C), GFAPdelta (D–F) while only a few cells express doublecortin (G–I). Bar size is 50 μm in all images (bar in C applies to A–F; bar in I applies to G–I).
Fig. S3. HuCNS-SC phagocytose ROS in vitro. (A) The vast majority of HuCNS-SC express the αvβ5 integrin. (B) Representative images of ARPE19 (top panels) and HuCNS-SC (bottom panels) containing internalized alexa488-labeled ROS. In the presence of antibodies against αvβ5, the amount of internalized ROS is decreased. Inserts show high-magnification merged images of phase contrast and Alexa488 fluorescence, highlighting internalized ROS. (C) Quantification of internalized fluorescence by pixel analysis for HuCNS-SC and ARPE-19, in the presence of anti-αvβ5 antibody or isotype matched control antibody (IgG); ROS, rod outer segment. Bars represent standard error of the mean.
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