These authors contributed equally to this study.
Thinking of Co2+-staining explant tissue or cultured cells? How to make it reliable and specific
Article first published online: 18 APR 2012
© 2012 The Authors. European Journal of Neuroscience © 2012 Federation of European Neuroscience Societies and Blackwell Publishing Ltd
European Journal of Neuroscience
Volume 35, Issue 8, pages 1201–1207, April 2012
How to Cite
Aurousseau, M. R. P., Osswald, I. K. and Bowie, D. (2012), Thinking of Co2+-staining explant tissue or cultured cells? How to make it reliable and specific. European Journal of Neuroscience, 35: 1201–1207. doi: 10.1111/j.1460-9568.2012.08042.x
- Issue published online: 18 APR 2012
- Article first published online: 18 APR 2012
- Received 7 December 2011, revised 4 January 2012, accepted 17 January 2012
- calcium permeability;
- cobalt staining;
- silver intensification;
Ca2+ and/or Zn2+ entry into neurons and glial cells is often a key step driving the processes of neurodevelopment and disease. As a result, a major pre-occupation of many neuroscientists has been in tracking down when and where nervous tissues express ion channels with appreciable divalent ion permeability. The cobalt (Co2+)-staining technique is one of the few techniques that allow a snapshot of the entire neuronal circuit, and selectively labels cells expressing divalent-permeable ion channels with a brown–black precipitate. Despite this, its use has been remarkably limited in the past decade. Reluctance to employ this approach has largely been related to an earlier concern with obtaining a reliable and reproducible means of visualizing transported Co2+. Here we show that recent advances have resolved these issues, opening this straightforward and valuable technique to a much larger neuroscience audience.