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Fig. S1. Traces without low-frequency filtering and including initial stimulation and latent period. (A) Recording arrangement. Signals from two optical detectors (marked as filled circles 1 and 2) and LFPs under three drug conditions are shown in C. The blue dashed lines in traces of C are zero baselines. Vertical scale: 2 × 10−3 of resting light intensity. Horizontal scale: 1 s. The solid blue lines in C mark the stimulus delivery time points. (B) Power spectrum (logarithmic scale) for optical signals during a 6 s episode averaged across all detectors of 10 slice experiments without low-frequency filtering. Note that there was no substantial difference in spectra between 0 and 5 Hz between these different pharmacological conditions. There is a substantial low-frequency component in the raw traces in C that are not affected by the different drug applications. We attribute these low frequencies to likely movement artifact, but some intrinsic signal components may also be present (not reflecting electrical activity). Note the latent period from stimulus to oscillation of about 1 s clearly shown in the LFP traces in C. Carb, carbachol; bic, bicuculline; stim, stimulus.

Fig. S2. Traces without low-frequency filtering with and without CGP52432. (A) Signals from two example optical detectors (marked as 1 and 2 in Fig. S1), along with LFPs, under two drug conditions. Vertical scale: 2 × 10−3 of resting light intensity. Horizontal scale: 1 s. The solid blue lines mark out the stimulus time points. (B) Traces of optical detectors 1 and 2 in A were filtered between 5 and 30 Hz and denoised. Vertical scale: 2 × 10−4 of resting light intensity. (C) Power spectrum (logarithmic scale) for optical signals during a 6 s episode averaged across all detectors of nine slice experiments without low-frequency filtering. The blue dashed lines in traces of A and B are zero baselines. Carb, carbachol; bic, bicuculline; CGP, CGP52432.

Fig. S3. Traces without low-frequency filtering with and without d-APV. (A) Signals from two example optical detectors (marked as 1 and 2 in Fig. S1), along with LFPs, under two drug conditions. Vertical scale: 2 × 10−3 of resting light intensity. Horizontal scale: 1 s. The vertical solid blue lines mark out the stimulus time points. (B) Traces of optical detectors 1 and 2 in A were filtered between 5 and 30 Hz and denoised. Vertical scale: 2 × 10−4 of resting light intensity. (C) Power spectrum (logarithmic scale) for optical signals during a 6-s episode averaged across all detectors of eight slice experiments without low-pass filtering below 5 Hz. The blue dashed lines in traces of A and B are zero baselines. Carb, carbachol; bic, bicuculline.

Fig. S4. Theta oscillations were eliminated by CNQX. (A) Recording arrangement,, with stimulation (stim) and local field potential (LFP) electrodes., with stimulation (stim) and local field potential (LFP) electrodes. Signals from one example optical detector (marked as a black filled circle in A) with and without CNQX are shown as 6 s traces in B. The blue horizontal lines are zero baselines. Vertical scale: 2 × 10−4 of resting light intensity. (C) Two sections from the temporal data (I and II) in B are displayed as a sequence of pseudocolor images. Note there were no propagating waves during the recording period after CNQX was added.

Movie S1. Ring waves evoked by 1 μm bicuculline/100 μm carbachol (AVI).

Movie S2. Plane waves evoked by 5 μm bicuculline/100 μm carbachol (AVI).

Movie S3. Spiral waves (AVI).

Movie S4. Ring waves and collisions evoked by 10 μm bicuculline/100 μm carbachol (AVI).

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EJN_8132_sm_FigS1.tif1134KSupporting info item
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EJN_8132_sm_FigS3.tif1053KSupporting info item
EJN_8132_sm_FigS4.tif1228KSupporting info item
EJN_8132_sm_movie_1Ringwaveevokedby1uMbic.mp4389KSupporting info item
EJN_8132_sm_movie_2planewavesevokedby5uMbic.mp4105KSupporting info item
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