Spatio-temporal control of neural activity in vivo using fluorescence microendoscopy

Authors

  • Yuichiro Hayashi,

    1. Department of Systems Biology, Osaka Bioscience Institute, Suita, Osaka 565-0874, Japan
    2. PRESTO, Japan Science and Technology Agency, Honcho, Kawaguchi, Saitama, Japan
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  • Yoshiaki Tagawa,

    1. Department of Biophysics, Kyoto University Graduate School of Science, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto, Japan
    2. Core Research Science and Technology, Japan Science and Technology Agency, Honcho, Kawaguchi, Saitama, Japan
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  • Satoshi Yawata,

    1. Department of Systems Biology, Osaka Bioscience Institute, Suita, Osaka 565-0874, Japan
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  • Shigetada Nakanishi,

    1. Department of Systems Biology, Osaka Bioscience Institute, Suita, Osaka 565-0874, Japan
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  • Kazuo Funabiki

    1. Department of Systems Biology, Osaka Bioscience Institute, Suita, Osaka 565-0874, Japan
    2. Core Research Science and Technology, Japan Science and Technology Agency, Honcho, Kawaguchi, Saitama, Japan
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Dr Y. Hayashi, 1Department of Systems Biology, as above.
E-mail: yhayashi@obi.or.jp

Abstract

Controlling neural activity with high spatio-temporal resolution is desired for studying how neural circuit dynamics control animal behavior. Conventional methods for manipulating neural activity, such as electrical microstimulation or pharmacological blockade, have poor spatial and/or temporal resolution. Algal protein channelrhodopsin-2 (ChR2) enables millisecond-precision control of neural activity. However, a photostimulation method for high spatial resolution mapping in vivo is yet to be established. Here, we report a novel optical/electrical probe, consisting of optical fiber bundles and metal electrodes. Optical fiber bundles were used as a brain-insertable endoscope for image transfer and stimulating light delivery. Light-induced activity from ChR2-expressing neurons was detected with electrodes bundled to the endoscope, enabling verification of light-evoked action potentials. Photostimulation through optical fiber bundles of transgenic mice expressing ChR2 in layer 5 cortical neurons resulted in single-whisker movement, indicating spatially restricted activation of neurons in vivo. The probe system described here and a combination of various photoactive molecules will facilitate studies on the causal link between specific neural activity patterns and behavior.

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