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Figure S1. GAD65-GFP+ interneurons preferentially express a variety of markers expressed in CGE-derived interneurons but not MGE-derived interneurons. At P21 GAD65-GFP+ interneurons in the somatosensory cortex rarely express markers of cortical interneuron subtypes derived from the medial ganglionic eminences (MGE) such as somatostatin (A) or parvalbumin (B) but preferentially express markers of interneuron subtypes derived from the caudal ganglionic eminences (GGE) such as VIP (C), reelin (D), NPY (E), calretinin (F). Graph showing that at P21 in upper (I-IV) (G) and lower cortical layers (V-VI) (G’) of the somatosensory cortex GAD65-GFP+ interneurons express markers for CGE-derived interneurons (calretinin, VIP, NPY, reelin) and only rarely markers for MGE-derived interneurons (PV, SST). SST, somatostatin, PV, parvalbumin, VIP, vasoactive intestinal peptide, NPY, neuropeptide Y. Scale bar: 100 μm  for A-B and 10 μm for C-F.

Movie S1. Activation of adra1 with cirazoline affects interneuron migration. Time-lapse movie showing migrating GAD65-GFP positive cells under control conditions ascending from the intermediate zone towards the cortical plate (white arrows). After adra1 activation (cirazoline 500 mM; blue arrows) cells are halted in their migration.

Movie S2. Activation of adra2 with medetomidine affects interneuron migration. Time-lapse movie showing migrating GAD65-GFP positive cells under control conditions ascending from the intermediate zone towards the cortical plate (white arrows). After adra2 activation (medetomidine 500 mM; blue arrows) cells are halted in their migration.

Movie S3. Activation of adra2 with guanfacine affects interneuron migration. Time-lapse movie showing migrating GAD65-GFP positive cells under control conditions ascending from the intermediate zone towards the cortical plate (white arrows). After adra2 activation (guanfacine 500 mM; blue arrows) cells are halted in their migration.

Movie S4. Long-term activation of adra2 with medetomidine affects interneuron migration. Time-lapse movie showing migrating GAD65-GFP positive cells under control conditions ascending from the intermediate zone towards the cortical plate (white arrows). After long-term adra2 activation (medetomidine 500 mM; blue arrows) cells are persistently halted in their migration.

Movie S5. Effects of adra2 activation on interneuron migration are reversible. Time-lapse movie showing migrating GAD65-GFP positive cells under control conditions ascending from the intermediate zone towards the cortical plate (white arrows). After adra2 activation (medetomidine 500 mM; light blue arrows) cells are halted in their migration but this effect is reversible after removal of the drug (dark blue arrows).

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