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Syntaxin 1 is required for DCC/Netrin-1-dependent chemoattraction of migrating neurons from the lower rhombic lip

Authors

  • Tiziana Cotrufo,

    1. Developmental Neurobiology and Regeneration Unit, Department of Cell Biology, Institute for Research in Biomedicine, Parc Cientific de Barcelona, Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), University of Barcelona, Barcelona, Spain
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  • Rosa María Andrés,

    1. Developmental Neurobiology and Regeneration Unit, Department of Cell Biology, Institute for Research in Biomedicine, Parc Cientific de Barcelona, Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), University of Barcelona, Barcelona, Spain
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  • Oriol Ros,

    1. Developmental Neurobiology and Regeneration Unit, Department of Cell Biology, Institute for Research in Biomedicine, Parc Cientific de Barcelona, Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), University of Barcelona, Barcelona, Spain
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  • Francesc Pérez-Brangulí,

    1. Developmental Neurobiology and Regeneration Unit, Department of Cell Biology, Institute for Research in Biomedicine, Parc Cientific de Barcelona, Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), University of Barcelona, Barcelona, Spain
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  • Ashraf Muhaisen,

    1. Developmental Neurobiology and Regeneration Unit, Department of Cell Biology, Institute for Research in Biomedicine, Parc Cientific de Barcelona, Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), University of Barcelona, Barcelona, Spain
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  • Giulia Fuschini,

    1. Developmental Neurobiology and Regeneration Unit, Department of Cell Biology, Institute for Research in Biomedicine, Parc Cientific de Barcelona, Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), University of Barcelona, Barcelona, Spain
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  • Ramón Martínez,

    1. Developmental Neurobiology and Regeneration Unit, Department of Cell Biology, Institute for Research in Biomedicine, Parc Cientific de Barcelona, Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), University of Barcelona, Barcelona, Spain
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  • Marta Pascual,

    1. Developmental Neurobiology and Regeneration Unit, Department of Cell Biology, Institute for Research in Biomedicine, Parc Cientific de Barcelona, Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), University of Barcelona, Barcelona, Spain
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  • Joan X. Comella,

    1. Cell Signaling and Apoptosis Laboratory, Institut de Recerca del Hospital Vall D’Hebron (VHIR) and Institut de Neurociències, Autonomous University of Barcelona, Bellaterra, Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Barcelona, Spain
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  • Eduardo Soriano

    1. Developmental Neurobiology and Regeneration Unit, Department of Cell Biology, Institute for Research in Biomedicine, Parc Cientific de Barcelona, Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), University of Barcelona, Barcelona, Spain
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Errata

This article is corrected by:

  1. Errata: Syntaxin 1 is required for DCC/Netrin-1-dependent chemoattraction of migrating neurons from the lower rhombic lip Volume 38, Issue 2, 2338, Article first published online: 14 July 2013

Dr E. Soriano, Cell and Developmental Biology Programme, IRB Barcelona, Científic de Barcelona, University of Barcelona, Baldiri Reixac 10, Barcelona E-08028, Spain.
E-mail: eduardo.soriano@irbbarcelona.org

Abstract

Directed cell migration and axonal guidance are essential steps in neural development that share many molecular mechanisms. The guidance of developing axons and migrating neurons is likely to depend on the precise control of plasmalemma turnover in selected regions of leading edges and growth cones, respectively. Previous results provided evidence of a signaling mechanism that couples chemotropic deleted in colorectal cancer (DCC)/Netrin-1 axonal guidance and exocytosis through Syntaxin1(Sytx1)/TI-VAMP SNARE proteins. Here we studied whether Netrin-1-dependent neuronal migration relies on a similar SNARE mechanism. We show that migrating neurons in the lower rhombic lip (LRL) express several SNARE proteins, and that DCC co-associates with Sytx1 and TI-VAMP in these cells. We also demonstrate that cleavage of Sytx1 by botulinum toxin C1 (BoNT/C1) abolishes Netrin-1-dependent chemoattraction of migrating neurons, and that interference of Sytx1 functions with shRNAs or Sytx1-dominant negatives disrupts Netrin-1-dependent chemoattraction of LRL neurons. These findings indicate that a Sytx1/DCC interaction is required for Netrin-1 guidance of migrating neurons, thereby highlighting a relationship between guidance signaling and SNARE proteins that regulate membrane turnover.

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