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Figure S1 Effects of elevated CO2 on plant biomass, total plant N pool, total plant biomass N concentration, and soil pH and moisture.

Figure S2 Effects of elevated CO2 on soil C and N dynamics.

Figure S3 DCA analyses of GeoChip 2.0 and PLFA data.

Figure S4 The abundance of 16S rRNA gene sequences at the phylum level at aCO2 and eCO2 and the significantly changed genera under eCO2.

Figure S5 Normalized average signal intensity of the significantly changed nifH genes and other top 10 abundant nifH sequences detected by GeoChip 3.0.

Figure S6 Normalized average signal intensity of detected key gene families involved in the N cycling under both CO2 conditions.

Figure S7 The normalized average signal intensity of the detected key functional genes involved in P cycling (A), and methane production and oxidation (B) under ambient CO2 and elevated CO2.

Table S1 Overall microbial community diversity and the number of detected genes involved in carbon, nitrogen, phosphorus, and sulfur cycling under ambient CO2 and elevated CO2.

Table S2 Simple Mantel tests and partial CCA analyses of correlations between key functional gene categories and environmental and plant variables.

Data S1 Materials and methods.

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