The use of DGGE analyses to explore eastern Mediterranean and Red Sea marine picophytoplankton assemblages
Article first published online: 17 MAR 2004
Volume 6, Issue 5, pages 528–534, May 2004
How to Cite
Zeidner, G. and Béjà, O. (2004), The use of DGGE analyses to explore eastern Mediterranean and Red Sea marine picophytoplankton assemblages. Environmental Microbiology, 6: 528–534. doi: 10.1111/j.1462-2920.2004.00599.x
- Issue published online: 17 MAR 2004
- Article first published online: 17 MAR 2004
- Received 23 September, 2003; revised 2 December, 2003; accepted 3 December, 2003.
In vast areas of the oceans, most marine photosynthetic production is performed by cells smaller than 2–3 µm (picoplankton). Here, we report on denaturing gradient gel electrophoresis (DGGE) analyses of naturally occurring marine oxygenic picophytoplankton using the conserved photosynthetic psbA gene. The psbA gene proved to be a good indicator for picophytoplankton presence and was shown to work with DGGE. The DGGE results show the distribution of photosynthetic marine groups belonging to cyanobacteria and the eukaryotic prasinophytes (green algae) in the Red and eastern Mediterranean Seas in the seasons examined. The present study demonstrates the value of DGGE as a tool for rapid analyses of natural marine communities of picophytoplankton.