Diversity of functional genes of methanogens, methanotrophs and sulfate reducers in deep-sea hydrothermal environments

Authors

  • Olivier Nercessian,

    Corresponding author
    1. UMR 6539, Laboratoire des Sciences de l’Environnement Marin, Centre National de la Recherche Scientifique and Université de Bretagne Occidentale, Institut Universitaire Européen de la Mer, Technopôle Brest-Iroise, Place Nicolas Copernic, 29280 Plouzané, France.
      *E-mail jeanthon@univ-brest.fr and nerceo@u.washington.edu; Tel. (+33) 298 498751; Fax (+33) 298 498705.
    Search for more papers by this author
    • Present address: Department of Chemical Engineering, Box 352125, University of Washington, Seattle, WA 98195, USA.

  • Nadège Bienvenu,

    1. UMR 6539, Laboratoire des Sciences de l’Environnement Marin, Centre National de la Recherche Scientifique and Université de Bretagne Occidentale, Institut Universitaire Européen de la Mer, Technopôle Brest-Iroise, Place Nicolas Copernic, 29280 Plouzané, France.
    Search for more papers by this author
  • David Moreira,

    1. Unité d’Ecologie, Systématique et Evolution, UMR 8079, Centre National de la Recherche Scientifique and Université Parid-Sud, 91405 Orsay Cedex, France.
    Search for more papers by this author
  • Daniel Prieur,

    1. UMR 6539, Laboratoire des Sciences de l’Environnement Marin, Centre National de la Recherche Scientifique and Université de Bretagne Occidentale, Institut Universitaire Européen de la Mer, Technopôle Brest-Iroise, Place Nicolas Copernic, 29280 Plouzané, France.
    Search for more papers by this author
  • Christian Jeanthon

    Corresponding author
    1. UMR 6539, Laboratoire des Sciences de l’Environnement Marin, Centre National de la Recherche Scientifique and Université de Bretagne Occidentale, Institut Universitaire Européen de la Mer, Technopôle Brest-Iroise, Place Nicolas Copernic, 29280 Plouzané, France.
      *E-mail jeanthon@univ-brest.fr and nerceo@u.washington.edu; Tel. (+33) 298 498751; Fax (+33) 298 498705.
    Search for more papers by this author

*E-mail jeanthon@univ-brest.fr and nerceo@u.washington.edu; Tel. (+33) 298 498751; Fax (+33) 298 498705.

Summary

To contribute to the identification of methanogens, methanotrophs and sulfate-reducing bacteria (SRB) in microbial communities from the 13°N (East Pacific Rise) and Rainbow (Mid-Atlantic Ridge) hydrothermal vent fields, we investigated the diversity of mcrA, pmoA and dsrAB genes sequences. Clone libraries were obtained using DNA isolated from fragments of diffuse vents, sediment and in situ samplers. The clones were categorized by restriction fragment length polymorphism, and representatives of each group were sequenced. Sequences were related to that of hyperthermophilic (order Methanopyrales and family Methanocaldococcaceae), thermophilic and mesophilic (family Methanococcaceae) methanogens, thermophilic (proposed genus ‘Methylothermus’) and mesophilic type I methanotrophs, and hyperthermophilic (order Archaeoglobales), thermophilic (order Thermodesulfobacteriales) and mesophilic (family Desulfobulbaceae) SRB. Several of the obtained sequences were distantly related to the genes of cultivated organisms, providing evidence of the existence of novel lineages in the three functional groups. This study provides for the first time an insight into the diversity of several functional genes of deep-sea hydrothermal system microorganisms.

Ancillary