Present address: Department of Chemical Engineering, Box 352125, University of Washington, Seattle, WA 98195, USA.
Diversity of functional genes of methanogens, methanotrophs and sulfate reducers in deep-sea hydrothermal environments
Article first published online: 2 SEP 2004
Volume 7, Issue 1, pages 118–132, January 2005
How to Cite
Nercessian, O., Bienvenu, N., Moreira, D., Prieur, D. and Jeanthon, C. (2005), Diversity of functional genes of methanogens, methanotrophs and sulfate reducers in deep-sea hydrothermal environments. Environmental Microbiology, 7: 118–132. doi: 10.1111/j.1462-2920.2004.00672.x
- Issue published online: 2 SEP 2004
- Article first published online: 2 SEP 2004
- Received 17 February, 2004; revised 25 May, 2004; accepted 25 May, 2004.
To contribute to the identification of methanogens, methanotrophs and sulfate-reducing bacteria (SRB) in microbial communities from the 13°N (East Pacific Rise) and Rainbow (Mid-Atlantic Ridge) hydrothermal vent fields, we investigated the diversity of mcrA, pmoA and dsrAB genes sequences. Clone libraries were obtained using DNA isolated from fragments of diffuse vents, sediment and in situ samplers. The clones were categorized by restriction fragment length polymorphism, and representatives of each group were sequenced. Sequences were related to that of hyperthermophilic (order Methanopyrales and family Methanocaldococcaceae), thermophilic and mesophilic (family Methanococcaceae) methanogens, thermophilic (proposed genus ‘Methylothermus’) and mesophilic type I methanotrophs, and hyperthermophilic (order Archaeoglobales), thermophilic (order Thermodesulfobacteriales) and mesophilic (family Desulfobulbaceae) SRB. Several of the obtained sequences were distantly related to the genes of cultivated organisms, providing evidence of the existence of novel lineages in the three functional groups. This study provides for the first time an insight into the diversity of several functional genes of deep-sea hydrothermal system microorganisms.