Culturable marine actinomycete diversity from tropical Pacific Ocean sediments
Article first published online: 9 JUN 2005
Volume 7, Issue 7, pages 1039–1048, July 2005
How to Cite
Jensen, P. R., Gontang, E., Mafnas, C., Mincer, T. J. and Fenical, W. (2005), Culturable marine actinomycete diversity from tropical Pacific Ocean sediments. Environmental Microbiology, 7: 1039–1048. doi: 10.1111/j.1462-2920.2005.00785.x
- Issue published online: 9 JUN 2005
- Article first published online: 9 JUN 2005
- Received 14 May, 2004; revised 16 November, 2004; accepted 17 November, 2004.
Actinomycetes were cultivated using a variety of media and selective isolation techniques from 275 marine samples collected around the island of Guam. In total, 6425 actinomycete colonies were observed and 983 (15%) of these, representing the range of morphological diversity observed from each sample, were obtained in pure culture. The majority of the strains isolated (58%) required seawater for growth indicating a high degree of marine adaptation. The dominant actinomycete recovered (568 strains) belonged to the seawater-requiring marine taxon ‘Salinospora’, a new genus within the family Micromonosporaceae. A formal description of this taxon has been accepted for publication (Maldonado et al., 2005) and includes a revision of the generic epithet to Salinispora gen. nov. Members of two major new clades related to Streptomyces spp., tentatively called MAR2 and MAR3, were cultivated and appear to represent new genera within the Streptomycetaceae. In total, five new marine phylotypes, including two within the Thermomonosporaceae that appear to represent new taxa, were obtained in culture. These results support the existence of taxonomically diverse populations of phylogenetically distinct actinomycetes residing in the marine environment. These bacteria can be readily cultured using low nutrient media and represent an unexplored resource for pharmaceutical drug discovery.