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Appendix S1. PCR-RFLP analysis of nifH sequences for soils below tufts of three different grass species (Arrhenatherum elatius; Holcus lanatus; Dactylis glomerata) on intensively and extensively managed plots with restriction enzymes MnlI and NdeII.

Appendix S2. PCR-RFLP analysis of narG sequences for soils below tufts of three different grass species (Arrhenatherum elatius; Holcus lanatus; Dactylis glomerata) on intensively and extensively managed plots.

Appendix S3. DGGE analysis of 16S rRNA gene sequences of ammonia oxidizers for soils below tufts of three different grass species (Arrhenatherum elatius; Holcus lanatus; Dactylis glomerata) on intensively and extensively managed plots.

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