Residual polymerase activity-induced bias in terminal restriction fragment length polymorphism analysis
Article first published online: 1 NOV 2006
Volume 9, Issue 2, pages 555–559, February 2007
How to Cite
Hartmann, M., Enkerli, J. and Widmer, F. (2007), Residual polymerase activity-induced bias in terminal restriction fragment length polymorphism analysis. Environmental Microbiology, 9: 555–559. doi: 10.1111/j.1462-2920.2006.01169.x
- Issue published online: 1 NOV 2006
- Article first published online: 1 NOV 2006
- Received 13 September, 2006; accepted 20 September, 2006.
Residual activity of polymerase chain reaction DNA polymerases in restriction analyses strongly affected genetic profiling based on terminal restriction fragment length polymorphisms. Artificial fragment sizes produced as a result of 5′-overhang restriction site fill-in and addition of a terminal A may bias genetic profiling and genotyping of microbial communities. Efficient removal of polymerases retained original fragment sizes and significantly reduced this profiling bias in soil bacterial communities.