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The use of functional genomics for the identification of a gene cluster encoding for the biosynthesis of an antifungal tambjamine in the marine bacterium Pseudoalteromonas tunicata

Authors

  • Catherine Burke,

    1. School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, Australia, and Centre for Marine Biofouling and Bio-Innovation, The University of New South Wales, Sydney 2052, Australia.
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  • Torsten Thomas,

    1. School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, Australia, and Centre for Marine Biofouling and Bio-Innovation, The University of New South Wales, Sydney 2052, Australia.
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  • Suhelen Egan,

    1. School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, Australia, and Centre for Marine Biofouling and Bio-Innovation, The University of New South Wales, Sydney 2052, Australia.
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  • Staffan Kjelleberg

    Corresponding authorSearch for more papers by this author

*E-mail S.Kjelleberg@unsw.edu.au; Tel. (+61) 2 93852102; Fax (+61) 2 93851591.

Summary

A large insert library was created in Escherichia coli from the DNA of the surface-associated marine bacterium Pseudoalteromonas tunicata. Screening of the library for antifungal activity resulted in the detection and identification of a large gene cluster encoding for the biosynthesis of an antifungal tambjamine. A biosynthetic pathway has been proposed based on analysis and annotation of the gene cluster.

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