Present address: Genetrix S.L. Calle Marconi, 1. Parque Tecnológico de Madrid, 28760 Tres Cantos, Madrid, Spain.
An oligonucleotide prokaryotic acidophile microarray: its validation and its use to monitor seasonal variations in extreme acidic environments with total environmental RNA
Version of Record online: 30 JAN 2008
© 2008 The Authors
Volume 10, Issue 4, pages 836–850, April 2008
How to Cite
Garrido, P., González-Toril, E., García-Moyano, A., Moreno-Paz, M., Amils, R. and Parro, V. (2008), An oligonucleotide prokaryotic acidophile microarray: its validation and its use to monitor seasonal variations in extreme acidic environments with total environmental RNA. Environmental Microbiology, 10: 836–850. doi: 10.1111/j.1462-2920.2008.01477.x
- Issue online: 30 JAN 2008
- Version of Record online: 30 JAN 2008
- Received 14 May, 2007; accepted 19 September, 2007.
Fig. S1. Theoretical prediction for positive specific hybridization for several species of bacteria and archaea relevant for AMD environments. The number of mismatches for each probe (left vertical) with the corresponding target in the 16SrRNA of each strain (horizontal) is indicated. +, perfect match; 1–4, one to four mismatches; blank, more than four mismatches. Light green indicates expected positive reactions even with one or more mismatches under low stringent hybridization conditions.
Fig. S2. PAM results obtained after hybridization with 16S rRNA gene amplified from environmental samples for comparison with other molecular ecology techniques (see text and Table 2). Scanned images and histograms with the signal intensities of the spots obtained from sample RT1 (A), RT7 (B) or RT8 (C). Only positive spots and some of the negative ones are represented in the histograms. See Fig. 1 for the identification of each probe on the microarray.
Table S1. Oligonucleotide probes used for FISH.
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