A microdiversity study of anammox bacteria reveals a novel Candidatus Scalindua phylotype in marine oxygen minimum zones
Article first published online: 28 MAY 2008
© 2008 The Authors. Journal compilation © 2008 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 10, Issue 11, pages 3106–3119, November 2008
How to Cite
Woebken, D., Lam, P., Kuypers, M. M. M., Naqvi, S. W. A., Kartal, B., Strous, M., Jetten, M. S. M., Fuchs, B. M. and Amann, R. (2008), A microdiversity study of anammox bacteria reveals a novel Candidatus Scalindua phylotype in marine oxygen minimum zones. Environmental Microbiology, 10: 3106–3119. doi: 10.1111/j.1462-2920.2008.01640.x
- Issue published online: 30 SEP 2008
- Article first published online: 28 MAY 2008
- Received 15 November, 2007; accepted 19 March, 2008.
Vol. 12, Issue 8, 2384, Article first published online: 4 AUG 2010
Table S1. 16S rRNA and ITS targeting probes specific for the ‘Scalindua cluster 2/Arabian Sea cluster’. The 16S rRNA probe Sarabica292 was designed to target the entire AS cluster. In order to improve the discrimination against sequences from the Peruvian and Namibian OMZs which have two mismatches at the 3′ end of the probe, a competitor is proposed for this probe. Probe Sarabica456 comprises 91% of the Arabian Sea cluster sequences, as it does not detect sequence EU048621 and two sequences from the Arabian Sea. Probes Sarabica1 and Sarabica2 target the ITS region, Sarabica1 fully matching to all sequences, whereas Sarabica2 only detects 94% of the sequences. Two sequences from the Arabian Sea are not targeted by Sarabica2. Furthermore, a competitor was designed for Sarabica2 to exclude sequences from the Peruvian OMZ that had only one mismatch with this probe.
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