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Table S1. 16S rRNA and ITS targeting probes specific for the ‘Scalindua cluster 2/Arabian Sea cluster’. The 16S rRNA probe Sarabica292 was designed to target the entire AS cluster. In order to improve the discrimination against sequences from the Peruvian and Namibian OMZs which have two mismatches at the 3′ end of the probe, a competitor is proposed for this probe. Probe Sarabica456 comprises 91% of the Arabian Sea cluster sequences, as it does not detect sequence EU048621 and two sequences from the Arabian Sea. Probes Sarabica1 and Sarabica2 target the ITS region, Sarabica1 fully matching to all sequences, whereas Sarabica2 only detects 94% of the sequences. Two sequences from the Arabian Sea are not targeted by Sarabica2. Furthermore, a competitor was designed for Sarabica2 to exclude sequences from the Peruvian OMZ that had only one mismatch with this probe.

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