Diversity of ammonium-oxidizing bacteria in a granular sludge anaerobic ammonium-oxidizing (anammox) reactor
Article first published online: 9 MAY 2008
© 2008 The Authors. Journal compilation © 2008 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 10, Issue 11, pages 3130–3139, November 2008
How to Cite
Quan, Z.-X., Rhee, S.-K., Zuo, J.-E., Yang, Y., Bae, J.-W., Park, J. R., Lee, S.-T. and Park, Y.-H. (2008), Diversity of ammonium-oxidizing bacteria in a granular sludge anaerobic ammonium-oxidizing (anammox) reactor. Environmental Microbiology, 10: 3130–3139. doi: 10.1111/j.1462-2920.2008.01642.x
- Issue published online: 30 SEP 2008
- Article first published online: 9 MAY 2008
- Received 7 September, 2007; accepted 19 March, 2008.
The ammonium-oxidizing microbial community was investigated in a granular sludge anaerobic ammonium-oxidizing (anammox) reactor that was operated for about 1 year with high anaerobic ammonium oxidation activity (up to 0.8 kg NH4+-N m−3 day−1). A Planctomycetales-specific 16S rRNA gene library was constructed to analyse the diversity of the anaerobic ammonium-oxidizing bacteria (AnAOB). Most of the specifically amplified sequences (15/16) were similar to each other (> 99%) but were distantly related to all of the previously recognized sequences (< 94%), with the exception of an unclassified anammox-related clone, KSU-1 (98%). An ammonia monooxygenase (amoA) gene library was also analysed to investigate the diversity of ‘aerobic’ ammonium-oxidizing bacteria (AAOB) from the β-Proteobacteria. Most of the amoA gene fragments (53/55) clustered in the Nitrosomonas europaea–Nitrosococcus mobilis group which has been reported to prevail under oxygen-limiting conditions. The quantitative results from real-time polymerase chain reaction (PCR) amplification showed that the dominant AnAOB comprised approximately 50% of the total bacterial 16S rRNA genes in the reactor, whereas the AAOB of β-Proteobacteria represented only about 3%. A large fragment (4008 bp) of the rRNA gene cluster of the dominant AnAOB (AS-1) in this reactor sludge was sequenced and compared with sequences of other Planctomycetales including four anammox-related candidate genera. The partial sequence of hydrazine-oxidizing enzyme (hzo) of dominant AnAOB was also identified using new designed primers. Based on this analysis, we propose to tentatively name this new AnAOB Candidatus‘Jettenia asiatica’.