Insights on Escherichia coli biofilm formation and inhibition from whole-transcriptome profiling


  • Thomas K. Wood

    Corresponding author
    1. Artie McFerrin Department of Chemical Engineering,
    2. The Department of Biology and
    3. The Zachry Department of Civil Engineering, 220 Jack E. Brown Building, Texas A & M University, College Station, TX 77843-3122, USA.
      *E-mail; Tel. (+1) 979 862 1588; Fax (+1) 979 865 6446.
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*E-mail; Tel. (+1) 979 862 1588; Fax (+1) 979 865 6446.


Biofilms transform independent cells into specialized cell communities. Here are presented some insights into biofilm formation ascertained with the best-characterized strain, Escherichia coli. Investigations of biofilm formation and inhibition with this strain using whole-transcriptome profiling coupled to phenotypic assays, in vivo DNA binding studies and isogenic mutants have led to discoveries related to the role of stress, to the role of intra- and interspecies cell signalling, to the impact of the environment on cell signalling, to biofilm inhibition by manipulating cell signalling, to the role of toxin/antitoxin genes in biofilm formation, and to the role of small RNAs on biofilm formation and dispersal. Hence, E. coli is an excellent resource for determining paradigms in biofilm formation and biofilm inhibition.