Four newly isolated fuselloviruses from extreme geothermal environments reveal unusual morphologies and a possible interviral recombination mechanism
Article first published online: 23 JUL 2009
© 2009 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Environmental Viruses: Shaping the biosphere. Guest Editors: Forest Rohwer, David Prangishvili and Debbie Lindell
Volume 11, Issue 11, pages 2849–2862, November 2009
How to Cite
Redder, P., Peng, X., Brügger, K., Shah, S. A., Roesch, F., Greve, B., She, Q., Schleper, C., Forterre, P., Garrett, R. A. and Prangishvili, D. (2009), Four newly isolated fuselloviruses from extreme geothermal environments reveal unusual morphologies and a possible interviral recombination mechanism. Environmental Microbiology, 11: 2849–2862. doi: 10.1111/j.1462-2920.2009.02009.x
- Issue published online: 27 OCT 2009
- Article first published online: 23 JUL 2009
- Received 2 April, 2009; accepted 18 June, 2009.
Spindle-shaped virus-like particles are abundant in extreme geothermal environments, from which five spindle-shaped viral species have been isolated to date. They infect members of the hyperthermophilic archaeal genus Sulfolobus, and constitute the Fuselloviridae, a family of double-stranded DNA viruses. Here we present four new members of this family, all from terrestrial acidic hot springs. Two of the new viruses exhibit a novel morphotype for their proposed attachment structures, and specific features of their genome sequences strongly suggest the identity of the host-attachment protein. All fuselloviral genomes are highly conserved at the nucleotide level, although the regions of conservation differ between virus-pairs, consistent with a high frequency of homologous recombination having occurred between them. We propose a fuselloviral specific mechanism for interviral recombination, and show that the spacers of the Sulfolobus CRISPR antiviral system are not biased to the highly similar regions of the fusellovirus genomes.